تاثیر تمرین هوازی بر فعالیت آنزیم‌های استیل‌کولین‌استراز و بوتریل‌کولین‌استراز سرم و تغییرات شاخص‌های خطر قلبی-متابولیکی زنان میانسال

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشیار فیزیولوژی ورزشی، دانشکده علوم تربیتی و روانشناسی، دانشگاه شهید مدنی آذربایجان، تبریز، ایران

2 دانشجوی دکتری فیزیولوژی ورزشی، گروه فیزیولوژی ورزشی و حرکات اصلاحی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه ارومیه، ارومیه، ایران.

چکیده

مقدمه و هدف: معمولا در وضعیت سندرم‌متابولیک، یک حالت التهابی مزمن درجه پایین حاکم است که به علت متمرکزشدن به اندام‌های داخلی تشخیص آن دشوار است. ولی افزایش فعالیت‌های استیل‌کولین‌استراز(ACHE)، بوتریل‌کولین‌استراز(BCHE) علاوه بر اینکه نشانگر قابل‌اعتماد این نوع التهاب هستند، همچنین به‌عنوان شاخص زودهنگام سندرم‌متابولیک نیز شناخته شده‌اند. بنابراین هدف ما بررسی تاثیر تمرین‌هوازی بر فعالیت ACHE و BCHE سرمی و شاخص­های خطر قلبی-متابولیک در زنان میانسال مبتلا به سندرم‌متابولیک یود.
روش­شناسی: مطالعه حاضر از نوع تجربی بود که در آن 25 (40-65 سال) زن چاق غیرفعال داوطلب دارای حداقل سه شاخص خطر سندرم متابولیک به­ طور تصادفی در دو گروه تجربی (13) و کنترل (12) تقسیم شدند. گروه تجربی به مدت هشت هفته در برنامه تمرین هوازی شامل راه رفتن و دویدن (3 بار درهفته)، به مدت 40دقیقه در هر جلسه با شدت 60 تا70 درصد از ضربان قلب ذخیره شرکت کردند. مقدار ACHE، BCHE سرم و سطوح شاخص‌های خطر متابولیک در طول مداخله با استفاده از آزمون تی همبسته به طور درون‌گروهی مقایسه شدند.
یافته­ها: در طول مداخله، تفاوت معنی‌داری در متغیرهای گروه کنترل مشاهده نشد(05/0<P). به‌دنبال تمرین هوازی، مقدار لیپوپروتئین پرچگال افزایش(001/0P=) و مقدار BCHE (008/0P=)، گلوکز خون(0001/0P=)، دور کمر(0001/0P=) و امتیاز z سندرم متابولیک(001/0P=) کاهش یافتند. ولی ACHE (34/0P=)، تری‌گلیسرید(052/0P=)، فشارخون متوسط سرخرگی(15/0P=)، تعداد عامل‌های خطر سندرم متابولیک(054/0P=)، پس از تمرین تغییر معنی‌داری نداشتند.
نتیجه­گیری: تمرین هوازی علاوه بر کاهش وخامت کلی سندرم‌متابولیک، به طور همزمان شدت التهاب سیستمیک را نیز کاهش می‌دهد که تصور می‌شود در جلوگیری از عواقب نامطلوب سندرم‌متابولیک بسیار موثر باشد. ولی به نظر می رسد که تفسیر دقیق نتایج ACHE نیازمند بررسی گلبولهای قرمز به جای سرم در تحقیقات آینده است.

کلیدواژه‌ها


عنوان مقاله [English]

The Effects of Aerobic Training on Blood ACHE and BCHE Activities and cardiometabolic Risk Factors Level in Midlife Women

نویسندگان [English]

  • Karim Azali -Alamdari 1
  • Yousef Saberi 2
1 1Associated Professor of Exercise Physiology, Faculty of Education and Psychology, Azarbaijan Shahid Madani University, Tabriz, Iran
2 Ph.D. student of Exercise Physiology, Faculty of Sport Sciences, Urmia University, Urmia, Iran..
چکیده [English]

Background&Purpose: There is a low grade inflammatory state in patients with metabolic syndrome (Mets) with internalization into deeper body organs which had made its diagnosis even more difficult. However, the activities of ACHE and BCHE were found as early indicators of Mets in addition to be as reliable markers of such inflammation. Therefore, we investigated the effects of aerobic training on serum ACHE and BCHE activities and cardiometabolic risk factors in middle aged women with Mets.
Methodology: The present study was an experimental study in which 25 volunteers (aged 40-65) who were volunteered for obesity with at least three indicators of metabolic syndrome were screened and randomly divided into experimental and control groups (12). The experimental group participated in the aerobic exercise program for walking and running (3 times a week) for 40 minutes and for 60 to 70 percent of maximum heart rate for 40 minutes. Data were analyzed by using t-test to compare the amount of acetylcholinesterase, butylcolinasease and metabolic syndrome indices between pre-test and post-test.
Results: Following to aerobic training, the plasma HDL (P=0.001) were increased and the serum BCHE activity (P=0.008), blood sugar (P=0.001), waist circumference (P=0.001) and Mets Z score (P=0.001) were decreased in the AT group.
Conclusion: After the aerobic exercise, the values of the boolean polysaccharide (p = 0.008), the high density lipoprotein (p = 0.001), the blood glucose (p = 0.0001), and the waist circumference (p = 0.0001) and the z score of the metabolic syndrome (p = 0.001) differed substantially. However, acetylcholinesterase (P = 0.34), triglyceride (p = 0.052), arterial blood pressure (p = 0.15), number of risk factors of metabolic syndrome (p = 0.054), after exercise were not significantly decreased.

کلیدواژه‌ها [English]

  • Aerobic training
  • Inflammation
  • ACHE
  • BCHE
  • Metabolic Syndrome
##Huang PL. A comprehensive definition for metabolic syndrome. Disease Models and Mechanisms. 2009;2(5-6):231-7.##Grundy SM, Brewer HB, Cleeman JI, Smith SC, Lenfant C. Definition of metabolic syndrome report of the National Heart, Lung, and Blood Institute/American Heart Association Conference on scientific issues related to definition. Circulation. 2004;109(3):433-8.##Azali Alamdari K, Ghorbanian B. Effect of aerobic training on serum adiponectin and ctrp-3 in males with metabolic syndrome. Iranian Journal of Endocrinology and Metabolism. 2017;18(5):368-77.##Palaniappan L, Carnethon MR, Wang Y, Hanley AJ, Fortmann SP, Haffner SM, et al. Predictors of the Incident Metabolic Syndrome in Adults The Insulin Resistance Atherosclerosis Study. Diabetes Care. 2004;27(3):788-93.##Alberti K, Eckel RH, Grundy SM, Zimmet PZ, Cleeman JI, Donato KA, et al. Harmonizing the metabolic syndrome a joint interim statement of the international diabetes federation task force on epidemiology and prevention; national heart, lung, and blood institute; American heart association; world heart federation; international atherosclerosis society; and international association for the study of obesity. Circulation. 2009;120(16):1640-5.##Athyros V, Elisaf M, Mikhailidis D. Inflammatory markers and the metabolic syndrome. Atherosclerosis. 2005;183(1):187-8.##Barzilay JI, Abraham L, Heckbert SR, Cushman M, Kuller LH, Resnick HE, et al. The relation of markers of inflammation to the development of glucose disorders in the elderly the Cardiovascular Health Study. Diabetes. 2001;50(10):2384-9.##Pradhan AD, Manson JE, Rifai N, Buring JE, Ridker PM. C-reactive protein, interleukin 6, and risk of developing type 2 diabetes mellitus. Jama. 2001;286(3):327-34.##Kaur J. A comprehensive review on metabolic syndrome. Cardiology research and practice. 2014;2014.##Zandi PP, Anthony JC, Hayden KM, Mehta K, Mayer L, Breitner JC. Reduced incidence of AD with NSAID but not H2 receptor antagonists The Cache County Study. Neurology. 2002;59(6):880-6.##Das UN. Acetylcholinesterase and butyrylcholinesterase as possible markers of low-grade systemic inflammation. Medical Science Monitor. 2007;13(12):RA214-RA21.##Das UN. Clinical laboratory tools to diagnose inflammation. Advances in Clinical Chemistry. 2006;41:189-231.##Kawashima K, Fujii T. The lymphocytic cholinergic system and its contribution to the regulation of immune activity. Life sciences. 2003;74(6):675-96.##Fried SK, Bunkin DA, Greenberg AS. Omental and subcutaneous adipose tissues of obese subjects release interleukin-6: depot difference and regulation by glucocorticoid 1. The Journal of Clinical Endocrinology & Metabolism. 1998;83(3):847-50.##Sridhar G, Nirmala G, Apparao A, Madhavi A, Sreelatha S, Rani JS, et al. Serum butyrylcholinesterase in type 2 diabetes mellitus: a biochemical and bioinformatics approach. Lipids in health and disease. 2005;4(1):1.##Starkie R, Ostrowski SR, Jauffred S, Febbraio M, Pedersen BK. Exercise and IL-6 infusion inhibit endotoxin-induced TNF-α production in humans. The FASEB Journal. 2003;17(8):884-6.##Routledge FS, Campbell TS, McFetridge-Durdle JA, Bacon SL. Improvements in heart rate variability with exercise therapy. Canadian Journal of Cardiology. 2010;26(6):303-12.##Tracey KJ. Reflex control of immunity. Nature Reviews Immunology. 2009;9(6):418-28.##Czura C, Tracey K. Autonomic neural regulation of immunity. Journal of internal medicine. 2005;257(2):156-66.##Cornelissen VA, Fagard RH. Effects of endurance training on blood pressure, blood pressure–regulating mechanisms, and cardiovascular risk factors. Hypertension. 2005;46(4):667-75.##Vaughan C, Schoo A, Janus ED, Philpot B, Davis-Lameloise N, Lo SK, et al. The association of levels of physical activity with metabolic syndrome in rural Australian adults. BMC Public Health. 2009;9(1):1.##Milano GE, Leite N, Chaves TJ, Milano GE, Souza RLRd, Alle LF. Butyrylcholinesterase activity and cardiovascular risk factors in obese adolescents submitted to an exercise program. Arquivos Brasileiros de Endocrinologia & Metabologia. 2013;57(7):533-7.##Silva IM, Leite N, Boberg D, Chaves TJ, Eisfeld GM, Eisfeld GM, et al. Effects of physical exercise on butyrylcholinesterase in obese adolescents. Genetics and Molecular Biology. 2012;35(4):741.##Mottillo S, Filion KB, Genest J, Joseph L, Pilote L, Poirier P, et al. The metabolic syndrome and cardiovascular risk: a systematic review and meta-analysis. Journal of the American College of Cardiology. 2010;56(14):1113-32.##She J, Nakamura H, Makino K, Ohyama Y, Hashimoto H. Selection of suitable maximum-heart-rate formulas for use with Karvonen formula to calculate exercise intensity. International Journal of Automation and Computing. 2015;12(1):62-9.##Tvarijonaviciute A, Ceron JJ, Tecles F. Acetylcholinesterase and butyrylcholinesterase activities in obese Beagle dogs before and after weight loss. Veterinary clinical pathology. 2013;42(2):207-11.##Tecles F, Subiela SM, Bernal L, Cerón J. Use of whole blood for spectrophotometric determination of cholinesterase activity in dogs. The Veterinary Journal. 2000;160(3):242-9.##Pizzi J, Furtado-Alle L, Schiavoni D, Lopes WA, da Rosa Silva L, Bono GF, et al. Reduction in Butyrylcholinesterase Activity and Cardiovascular Risk Factors in Obese Adolescents after 12-Weeks of High-Intensity Interval Training. Journal of Exercise Physiology Online. 2017;20(3).##Shenhar-Tsarfaty S, Sherf-Dagan S, Berman G, Webb M, Raziel A, Keidar A, et al. Obesity-related acetylcholinesterase elevation is reversed following laparoscopic sleeve gastrectomy. International Journal of Obesity. 2018:1.##Cazzola R, Rondanelli M, Trotti R, Cestaro B. Effects of weight loss on erythrocyte membrane composition and fluidity in overweight and moderately obese women. The Journal of nutritional biochemistry. 2011;22(4):388-92.##Boberg DR, Furtado-Alle L, Souza RL, Chautard-Freire-Maia EA. Molecular forms of butyrylcholinesterase and obesity. Genetics and Molecular Biology. 2010;33(3):452.##Massoulié J, Pezzementi L, Bon S, Krejci E, Vallette F-M. Molecular and cellular biology of cholinesterases. Progress in neurobiology. 1993;41(1):31-91.##Milano G, Leite N, Chaves T, Milano G, Souza R, Alle L. Butyrylcholinesterase activity and cardiovascular risk factors in obese adolescents submitted to an exercise program. Arquivos brasileiros de endocrinologia e metabologia. 2013;57(7):533.##Iwasaki T, Yoneda M, Nakajima A, Terauchi Y. Serum butyrylcholinesterase is strongly associated with adiposity, the serum lipid profile and insulin resistance. Internal Medicine. 2007;46(19):1633-9.##Chaves TJ, Leite N, Milano GE, Milano GE, Souza RLR, Chautard-Freire-Maia EA, et al. 116A and K BCHE gene variants associated with obesity and hypertriglyceridemia in adolescents from Southern Brazil. Chemico-biological interactions. 2013;203(1):341-3.##Lima JK, Leite N, Turek LV, Souza RLR, da Silva Timossi L, Osiecki ACV, et al. 1914G variant of BCHE gene associated with enzyme activity, obesity and triglyceride levels. Gene. 2013;532(1):24-6.##Chen VP, Gao Y, Geng L, Stout MB, Jensen MD, Brimijoin S. Butyrylcholinesterase deficiency promotes adipose tissue growth and hepatic lipid accumulation in male mice on high-fat diet. Endocrinology. 2016;157(8):3086-95.##Randell EW, Mathews MS, Zhang H, Seraj JS, Sun G. Relationship between serum butyrylcholinesterase and the metabolic syndrome. Clinical Biochemistry. 2005;38(9):799-805.##Arbel Y, Shenhar-Tsarfaty S, Waiskopf N, Finkelstein A, Halkin A, Revivo M, et al. Decline in serum cholinesterase activities predicts 2-year major adverse cardiac events. Molecular medicine. 2014;20(1):38.##Waiskopf N, Shenhar-Tsarfaty S, Soreq H. Serum Cholinesterase Activities as Biomarkers of Cardiac Malfunctioning. Biomarkers in Cardiovascular Disease. 2015:1-22.##Chen VP, Gao Y, Geng L, Brimijoin S. Butyrylcholinesterase gene transfer in obese mice prevents postdieting body weight rebound by suppressing ghrelin signaling. Proceedings of the National Academy of Sciences. 2017;114(41):10960-5.##Morton G, Cummings D, Baskin D, Barsh G, Schwartz M. Central nervous system control of food intake and body weight. Nature. 2006;443(7109):289.##Schopfer LM, Lockridge O, Brimijoin S. Pure human butyrylcholinesterase hydrolyzes octanoyl ghrelin to desacyl ghrelin. General and comparative endocrinology. 2015;224:61-8.##Zivkovic AR, Schmidt K, Sigl A, Decker SO, Brenner T, Hofer S. Reduced Serum Butyrylcholinesterase Activity Indicates Severe Systemic Inflammation in Critically Ill Patients. Mediators of Inflammation. 2015;2015.##Zivkovic AR, Tourelle KM, Brenner T, Weigand MA, Hofer S, Schmidt K. Reduced serum cholinesterase activity indicates splenic modulation of the sterile inflammation. journal of surgical research. 2017; 220:275-83.##Wärnberg J, Cunningham K, Romeo J, Marcos A. Physical activity, exercise and low-grade systemic inflammation. Proceedings of the Nutrition Society. 2010;69(3):400-6.##Santarpia L, Grandone I, Contaldo F, Pasanisi F. Butyrylcholinesterase as a prognostic marker: a review of the literature. Journal of cachexia, sarcopenia and muscle. 2013;4(1):31-9.##Azali Alamdari K, Rouhani H. The Response of Apelin and overall metabolic risk to Aerobic Training in Middle-aged Female patients with Metabolic Syndrome. Journal of applied exercise physiology. 2018; 13(26): 29-30.##Rohani H, Azali Alamdari K, Helali zadeh M. Effect of Aerobic Training on Overall Metabolic Risk and Indices Levels in Patients with Metabolic Syndrome: A Meta-Analysis Study. Sport Physiology. 2016;8(31):17-44.##Klancic T, Woodward L, Hofmann SM, Fisher EA. High density lipoprotein and metabolic disease: Potential benefits of restoring its functional properties. Mol Metab. 2016;5(5):321-7.##Kohrt WM, Obert KA, Holloszy JO. Exercise training improves fat distribution patterns in 60- to 70-year-old men and women. Journal of gerontology. 1992;47(4):M99-105.##Kissebah AH, Krakower GR. Regional adiposity and morbidity. Physiol Rev. 1994;74(4):761-811.##Babaei P, damirchi a, Azali Alamdari K. Effects of Endurance Training and Detraining on Serum BDNF and Memory Performance in Middle Aged Males with Metabolic Syndrome. Iranian Journal of Endocrinology and Metabolism. 2013;15(2):132-42.##Azali Alamdari K, Rohani H. Metabolic and endocrine adaptations of aerobic training in men with generalized stages of metabolic syndrome. Sport Physiology. 2015;7(27):149-66.##Damirchi A, Tehrani BS, Alamdari KA, Babaei P. Influence of Aerobic Training and Detraining on Serum BDNF, Insulin Resistance, and Metabolic Risk Factors in Middle-Aged Men Diagnosed With Metabolic Syndrome. Clinical Journal of Sport Medicine. 2014;24(6):513-8.##