آثار تمرین هوازی و محدودیت کالری بر چربی درون عضلانی، پروفایل لیپیدی و مقاومت به انسولین پس از 18 هفته رژیم غذایی پرچرب در موشهای صحرایی نر

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزش، گروه فیزیولوژی ورزش، دانشکده تربیت بدنی و علوم ورزشی،دانشگاه گیلان، رشت، ایران

2 استاد گروه فیزیولوژی ورزش، دانشکده علوم ورزشی، دانشگاه گیلان، رشت، ایران

چکیده

مقدمه و هدف: بی تحرکی با متابولیسم ناقص چربی درون عضلانی ارتباط دارد که منجر به تجمع متابولیت‌های اسید چرب و مقاومت به انسولین می شود. هدف از مطالعه حاضر، بررسی آثار تمرین هوازی و محدودیت کالری بر چربی درون عضلانی، پروفایل لیپیدی و مقاومت به انسولین پس از 18 هفته رژیم غذایی پرچرب در موش‌های صحرایی نر بود.
روش‌شناسی: نمونۀ آماری تحقیق حاضر را 48 سر موش صحرایی نر نژاد ویستار با میانگین وزن 6/12±5/194 گرم تشکیل دادند. نمونه­برداری بافت و خون پس از ناشتایی شبانه در هفته­های 18 و 28 صورت گرفت. پس از اندازه گیری وزن موش ها در هفته اول، موش‌ها رژیم غذایی پرچرب مصرف کردند. موش‌های تغذیه شده با غذای پرچرب پس از 18 هفته، به گروه­های کنترل و تعادل منفی انرژی شامل تمرین هوازی و محدودیت رژیم غذایی تقسیم شدند. گروه­های تعادل منفی انرژی به مدت 10 هفته، به ترتیب تحت تمرین هوازی (دویدن) با شدت70 تا 75 درصد حداکثر اکسیژن مصرفی و 25% محدودیت رژیم غذایی قرار گرفتند. سطوح تری گلیسرید لیپاز بافت چربی، دی و تری آسیل گلیسرول عضله نعلی و همچنین سطوح سرمی گلوکز، انسولین و نیمرخ لیپیدی اندازه­گیری شدند. شاخص مقاومت به انسولین نیز محاسبه گردید.
یافته‌ها: نتایج این مطالعه نشان داد که روش­های تعادل منفی انرژی سبب کاهش معنی‌دار سطوح دی آسیل گلیسرول عضله و سطوح سرمی انسولین، گلوکز و شاخص مقاومت به انسولین، در موش­های صحرایی چاق شد (05/0>P). همچنین تنها در گروه محدودیت رژیم غذایی کاهش معنی­دار تری آسیل گلیسرول درون عضلانی مشاهده شد (05/0>P). کاهش سطوح تری آسیل گلیسرول و کلسترول نیز فقط در گروه تمرین هوازی نشان داده شد (05/0>P).
نتیجه‌گیری: این مطالعه نشان داد که روش­های تعادل منفی انرژی حتی در زمان مصرف غذای پرچرب می­توانند سبب کاهش دی آسیل گلیسرول عضله اسکلتی شوند که تا حد زیادی با بهبود مقاومت به انسولین همسو است.

تازه های تحقیق

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کلیدواژه‌ها


عنوان مقاله [English]

Effects of Aerobic Training and Caloric Restriction on Intramuscular Lipid, Lipid Profile and Insulin Resistance after 18 Weeks High Fat Diet in Male Rat

نویسندگان [English]

  • Aboozar Jorbonian 1
  • Hamid Mohebbi 2
1 Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Guilan, Rasht, Iran
2 Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Guilan, Rasht, Iran
چکیده [English]

Background&Purpose: Physical inactivity is associated with dysregulation of intramuscular triglyceride (IMTG) metabolism leading to the accumulation of fatty acid metabolites and insulin resistance. The purpose of this study was to evaluate the effects of aerobic training and caloric restriction on intramuscular lipid metabolism, lipid profile and insulin resistance after 18 weeks high fat diet in male rat
Methodology: Study sample were consisted of forty eight Wistar male rats with average weight; 194.5±12.6 g. Blood and muscle samples were collected at, 18th and 28th weeks after an overnight fasting. After weight rats in first week, rats consume high-fat-diet. After 18 weeks High-fat-diet group randomly divided into control (C), calorie restriction (CR) and aerobic training (T) groups. Negative energy balance groups underwent an aerobic training (running) with intensity of 70 to 75% of VO2max, and %25 calorie restriction for 10 weeks. Adipose Triglyceride Lipase, Triacylglycerol (TAG) and diacylglycerol (DAG) Levels in soleus, insulin and glucose levels and lipid profile were measured. HOMA-IR was also calculated.
Results: The results of this study indicated that negative energy balance methods, decreased muscle DAG levels and serum concentrations of insulin, glucose, triacylglycerol and insulin resistance index in obese male rats (P≤0.05). Serum triacylglycerol and cholesterol levels decreased only in T group (P≤0.05).
Conclusion: This study indicated that negative energy balance methods can actually decrease TAG and DAG levels even with simultaneous consumption of high-fat-diet which is largely congruent with improvement in insulin resistance.

کلیدواژه‌ها [English]

  • Insulin Resistance
  • Negative-Energy-Balance
  • High-Fat-Diet
  • Triacylglycerol
  • Diacylglycerol

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