تازه های تحقیق
عنوان مقاله [English]
نویسندگان [English]چکیده [English]
Background&Purpose: Intensity endurance training induces oxidative stress in athleteis. The purpose of this study was to investigate the effects of curcumin supplementation and light resistance training on superoxide dismutase (SOD) activity of kidney tissues and malondialdehyde (MDA) as oxidative status level during 8 weeks of endurance training in male wistar rats.
Methodology: 33 Male Wistar rats (weight= 255.62±19.99 g, age= 8 weeks) were randomly divided in to 4 groups; Control (n=6), Endurance(n=9), Endurance+curcumin(n=9), Endurance+curcumin+resistance(n=9) after 1 week orentatioon period .Endurance training(8 weeks, 5 sessions per week) were carried out on an animal treadmill. The speed and duration of running were 10 m/min and 30 min at first week, respectively and reached to the 35 m/min and 70 min up to the last week. Resistance training (8 weeks, 2 sessions per week) were carried out on vertical ladder with extra weight (30-70% BW) fixed on tail. The animals received curcumin supplement by sub peritoneal injection (8 weeks, 3 sessions per week, 30 mg/kg.Bw). SOD activity and MDA levels of kidney tissues were measured by spectrophotometric. Analysis of variance was used to analysis of the data.
Results: The results of this study showed that strenuous endurance training decreases the SOD activity of and increases the MDA levels of kidney tissues (PConclusion: Based on findings of this study, it seems that curcumin supplementation and curcumin combined with light resistance training can prevent oxidative stress during strenuous endurance training.
##Belviranlı M, Gökbel H. 2006. Acute exercise induced oxidative stress and antioxidant changes. Eur J Gen Med. 3(3):126-31.##Powers SK, Jackson MJ. 2008 .Exercise-induced oxidative stress: cellular mechanisms and impact on muscle force production. Physiological reviews. 88(4):1243-76.##Salehi I, Mohammadi M, Asadi FA. 2009. The effect of treadmill exercise on antioxidant status in the hearts of the diabetic rats. Scientific Journal of Hamadan Univercity of Medical Sciences and Health Services. 16(2):20-25.##Teixeira V, Valente H, Casal S, Marques F, Moreira P. 2009. Antioxidant status, oxidative stress, and damage in elite trained kayakers and canoeists and sedentary controls. Int J Sport Nutr Exerc Metab. 19(5):443-56.##Gomez-Cabrera M-C, Domenech E, Viña J. 2008. Moderate exercise is an antioxidant: upregulation of antioxidant genes by training. Free Radical Biology and Medicine. 44(2):126-31.##Cavas L, Tarhan L. 2004. Effects of vitamin-mineral supplementation on cardiac marker and radical scavenging enzymes, and MDA levels in young swimmers. International journal of sport nutrition and exercise metabolism. 14(2):133-46.##Ji LL. 1999. Antioxidants and oxidative stress in exercise. Experimental Biology and medicine. 222(3):283-92.##Dehbashi Behbahani G, Hajhosseini R, Hoghoughirad L, Hedayati M. 2010. Sensitive chemiluminescence method for superoxide dismutase activity assaye. Kowsar Medical Journal. 15(3):129-33.##Mc Bride JM, Kraemer WJ. 1999. Free radicals, exercise and antioxidants. Med Sci Sports Exerc; 83-175: (2) 13.##Radak Z, Inoue A, Kizakit M, Ishis OH, Susuki K, Chin T, Noh O. 1995. Superoxide dismutase reduces oxidative damage in skeletal muscle of rats during exhaustive exercise. JAPPL Physiol; 79: 129-35.##Jahani. Gholamreza.et al. 1389. ”The effect of regular exercise on erythrocyte antioxidant enzyme activity and oxidative stress in elite soccer players. Iran University of Medical Sciences. Period XVII. No. 74. [Persian]##Halle M, Berg A, Baumstark M, Keul J. 1999. Association of physical fitness with LDL and HDL subfractions in young healthy men. International journal of sports medicine. 20(07):464-9.##Sacheck JM, Blumberg JB. Role of vitamin E and oxidative stress in exercise. 2001. Nutrition (Burbank, Los Angeles County, Calif). 17(10):809-14.##Gaeini A, Sheykh Aleslami VD, Alameh A, Ravasi A, Kordi M, Mogharnasi M. 2008. Effect of enduring training and a detraining period on lipid peroxidation and antioxidant system in wistar rats. Journal of Movement Science. 6(11):51-63.##Ahmadi Kani Gholzar. Farhad, SheikholeslamiVatani. Dariush, mojtahedi. Hossein, Marandi. Seyed Mohammad, Mashhadi. Masud. 1390."The role of resistance training and protein supplement's antioxidant status in overweight young men." Biological Sciences sport11. Pp. 103-121. [Persian]##GaeiniAbbasali, SheikholeslamiVataniDariush, AllamehAbdolamir, RavasiAliasghar, KordiMohammadreza, .1388. “The effect of endurance training and training on lipid peroxidation and anti-oxidation system Wistar rats ". Science of motor and sports. 11. 51- 63.[Persian]##Polidori MC, Mecocci P, Cherubini A, et al: 2000. Physical activity and oxidative stress during aging. Int J Sports Med 21:154-157. ##Kostka T, Drai J, Berthouze SE, et al: 1998. Physical activity, fitness and integrated antioxidant system in healthy active elderly women. Int J Sports Med. 19: 462-467.##Evelo CT, Palmen NG, Artur Y, et al. 1999. Changes in blood glutathione concentrations, and in erythrocyte. Glutathione reductase and glutathione S-transferase activity after running training and after participation in contests. Eur J Appl Physiol Occup physiol. 64: 354-358.##Fatouros JG, Jamurtas AZ, Villiotou V, et al. 2004. Oxidative stress responses in older men during endurance training and detraining. Med Sci Sports Exerc 36: 2065-2072.##Sharman JE, Geraghty DP, Shing CM, et al. 2004. Endurance exercise, plasma oxidation and cardiovascular risk. Acta Cardiol 59:636-642.##M.J. Bown, M.L. Nicholson, P.R. Bell, R.D. Sayers. 2001. Cytokines and inflammatory pathways in the pathogenesis of multiple organ failure following abdominal aortic aneurysm repair, Eur. J. Vasc. Endovasc. Surg. 22 (6) 485e495.##H.S. Khaira, S.R. Maxwell, H. Thomason, G.H. Thorpe, M.A. Green, et al. 1996. Antioxidant depletion during aortic aneurysm repair, Br. J. Surg. 83 (3) 401e403.##Hamdi P. 2011. The effects of gender and exercise on malondialdehyde, nitric oxide and total glutathione levels in rat liver. African Journal of Pharmacy and Pharmacology. 5(4):515-21.##Mahfouz MM, Zhou Q, Kummerow A. 2011. Effect of curcumin on LDL oxidation in vitro, and lipid peroxidation and antioxidant enzymes in cholesterol fed rabbits. International Journal for Vitamin and Nutrition Research. 81(6):378-91.##Vajragupta O, Boonchoong P, Watanabe H, Tohda M, Kummasud N, Sumanont Y. 2003. Manganese complexes of curcumin and its derivatives: evaluation for the radical scavenging ability and neuroprotective activity. Free Radical Biology and Medicine. 35(12):1632-44.##Aydin. Mehmet Salih, Ahmet Caliskan , Aydemir Kocarslan , Sezen Kocarslan , Ali Yildiz , Samil Günay , Emin Savik , Abdussemet Hazar , Funda Yalcin. 2014. Intraperitoneal curcumin decreased lung, renal and heart injury in abdominal aorta ischemia/reperfusion model in rat. 85-24.28##Farzanegi. Parvin, Saberi. Sadegh, Fakharian. Ali, Rasaee. Mohammad Javad, Dabidi Roshan. Valiollah. 2012. Combined Effects of Curcuma longa and Exercise Training on Kidney and Spleen Tissue Levels of Glutathione Peroxidase and Protein Carbonyl in Rats Exposed to Lead. 23. 52-58.##Pechter U, Ots M, Mesikepp S, et al. 2003. Beneficial effects of water-based exercise in patients with chronic kidney disease. Int J Rehabil Res 26:153-156.##Nabavi SF, Moghaddam AH, Eslami S, Nabavi SM. 2012. Protective effects of curcumin against sodium fluoride-induced toxicity in rat kidneys. Biol Trace Elem Res. 145(3):369-74##Lamina S, Ezema CI, Theresa AI, Anthonia EU. 2013. Effects of free radicals and antioxidants on exercise performance. Oxidants and Antioxidants in Medical Science. 2(2):83-91.##Roshan VD, Rahimi M, Shirinbayan V, Mahjoub S, Hosseinzadeh M. 2012. Protective effect of the combination of exercise and curcumin supplementation on cardiac system in rats exposed to lead. International Journal of Nutrition and Metabolism. 4(8):114-20.##Parise G, Brose AN, Tarnopolsky MA. 2005. Resistance exercise training decreases oxidative damage to DNA and increases cytochrome oxidase activity in older adults. Experimental gerontology. 40(3):173-80.##Gorzi A, Rajabi H, Gharakhanlou R, Azad A. 2013. Effects of Endurance Training on A12 Acetyl Cholinesterase Activity in Fast and Slow-Twitch Skeletal Muscles of Male Wistar Rats. Zahedan Journal of Research in Medical Sciences. 15(10):28-31.##Banaeifar AA, Gorzi A, Hedayati M, Nabiollahi Z, Rahmani Moghaddam N, Khantan M. 2011. Effect of an 8-week resistance training program on acetylcholinesterase activity in rat muscle. KAUMS Journal (FEYZ ). 15(4):316-21.##Daniel S, Limson JL, Dairam A, Watkins GM, Daya S. 2004. Through metal binding, curcumin protects against lead-and cadmium-induced lipid peroxidation in rat brain homogenates and against lead-induced tissue damage in rat brain. Journal of inorganic biochemistry. 98(2): 266-75.##Ono K, Kimura S, Nakano M, Naruse T. 1991. Detection of heterogeneity of Cu, Zn-superoxide dismutase with monoclonal antibodies and the establishment of a highly sensitive fluorescence sandwich enzyme-linked immunosorbent assay. FEBS Letters. 282(1):115-8.##Zhonghui Z, Xiaowei Z, Fang F. 2014. Ganoderma lucidum polysaccharides supplementation attenuates exercise-induced oxidative stress in skeletal muscle of mice. Saudi journal of biological sciences. 21(2):119-23.##Paoletti F, Aldinucci D, Mocali A, et al. 1986. A sensitive spectrophotometric method for the determination of superoxide dismutase activity in tissue extracts. Anal Biochem. 154:536.##Kaya H, Sezik M, Ozkaya O, et al. 2004. Lipid peroxidation at various estradiol concentrations in human circulation during ovarian stimulation with exogenous gonadotropins. Horm Metab Res. 36:693.## M.H. Somi, B. Hajipour, N.A. Asl, R. Estakhri, A.N. Azar, M.N. Zade, A.G. Haghjou, and A.M. Vatankhah. 2009. Pioglitazone Attenuates Ischemia/Reperfusion–Induced Liver Injury in Rats. Transplantation Proceedings. 41, 4105–4109##Taysi S, Oztasan N, Efe H, Polat M, Gumustekin K, Siktar E. 2008. Endurance training attenuates the oxidative stress due to acute exhaustive exercise in rat liver. Acta Physiologica Hungarica. 95(4):337-47.##Modiri MD, F Tanideh, N Mohammadi, M Firouzmand. 2014. The effects of short and middle times aerobic exercise with high intensities on ingredients antioxidant in female Sprague Dawley rats. Medical Journal of Mashhad univercity of medical sciences. 57(3):587-95.##Azizbeigi K, Stannard SR, Atashak S, Mosalman Haghighi M. 2014. Antioxidant enzymes and oxidative stress adaptation to exercise training: Comparison of endurance, resistance, and concurrent training in untrained males. Journal of Exercise Science & Fitness. 12(1):1-6.##Roshan VD, Assali M, Moghaddam AH, Hosseinzadeh M, Myers J. 2011. Exercise Training and Antioxidants Effects on Rat Heart Tissue Exposed to Lead Acetate. International journal of toxicology. 30(2):190-6.##Güzel NA, Hazar S, Erbas D. 2007. Effects of different resistance exercise protocols on nitric oxide, lipid peroxidation and creatine kinase activity in sedentary males. Journal of sports science & medicine. 6(4):417-22.##Tanwar V, Sachdeva J, Golechha M, Kumari S, Arya DS. 2010. Curcumin protects rat myocardium against isoproterenol-induced ischemic injury: attenuation of ventricular dysfunction through increased expression of hsp27 alongwith strengthening antioxidant defense system. Journal of cardiovascular pharmacology. 55(4):377-84.##