اثر مصرف مکمل کورکومین و تمرینات سبک مقاومتی به هنگام 8 هفته تمرین استقامتی بر میزان ظرفیت انتی آکسیدانی بافت کلیه موش‌های صحرایی نر ویستار

نوع مقاله: مقاله پژوهشی

نویسندگان

1 گروه فیزیولوژی ورزش، دانشکده ی تربیت بدنی و علوم ورزشی دانشگاه ارومیه، ارومیه، ایران

2 گروه تربیت بدنی و علوم ورزشی، دانشگاه زنجان، زنجان، ایران

چکیده

سابقه و هدف: تمرینات استقامتی شدید موجب فشار اکسایشی در ورزشکاران می­شود. هدف از پژوهش حاضر بررسی اثر مصرف مکمل کورکومین و تمرین مقاومتی سبک بر میزان فعالیت آنزیم سوپراکسید دیسموتاز (SOD) و سطوح مالون دی­آلدئید(MDA) در بافت کلیه موش­های نر ویستار طی 8 هفته تمرین استقامتی بود.
 مواد و روش­ها: 33 سر موش صحرایی نر ویستار (سن 8 هفته) پس از یک هفته آشناسازی به طور تصادفی به چهار گروه کنترل (6=n), تمرین استقامتی (9=n), تمرین استقامتی+ کورکومین (9=n) و تمرین استقامتی+کورکومین+تمرین مقاومتی (9=n) تقسیم شدند. تمرین استقامتی (8 هفته، 5 جلسه در هفته) روی نوارگردان مخصوص جوندگان انجام شد. سرعت و مدت دویدن در هفتۀ اول پژوهش به ترتیب 10 متر در دقیقه و 30 دقیقه بود و در هفتۀ آخر به سرعت 35 متر در دقیقه و زمان 70 دقیقه رسید. تمرین مقاومتی (8 هفته، 2 جلسه در هفته)  روی نردبان عمودی با بستن وزنه اضافی(با 30 الی 70 درصد وزن بدن) به دم موش­ها انجام شد. موش­های صحرایی مکمل کورکومین را به وسیله تزریق داخل صفاقی دریافت کردند (8 هفته، 3 جلسه در هفته، 30 میلی­گرم به ازای هر کیلوگرم از وزن بدن). میزان فعالیت آنزیم SOD و سطوح MDA در بافت کلیه با استفاده روش اسپکتروفتومتریک سنجیده شد. داده­ها به­وسیلۀ تحلیل واریانس، مورد تجزیه و تحلیل قرار گرفت.
یافته ها: نتایج حاصل از این پژوهش نشان داد که تمرین استقامتی شدید فعالیت آنزیم SOD دربافت کلیه را کاهش و نیز سطوح آنزیم MDA را افزایش می­دهد؛ درحالی­که مکمل کورکومین و ترکیب آن با تمرین مقاومتی سبک می­تواند از کاهش فعالیت آنزیمSOD و افزایش سطوح آنزیم MDA جلوگیری نماید (p نتیجه­ گیری: مکمل کورکومین و به­ویژه در ترکیب با تمرین مقاومتی سبک از فشار اکسایشی تمرین استقامتی شدید جلوگیری می­کند.

تازه های تحقیق

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کلیدواژه‌ها


عنوان مقاله [English]

The effect of curcumin supplementation accompanied by light resistance training during 8 weeks of endurance training on SOD and MDA levels Of kidney tissues in male Wistar rats

نویسندگان [English]

  • Asgar Tofigi 1
  • Ali Gorzi 2
  • Bahare Amin 1
چکیده [English]

Background&Purpose: Intensity endurance training induces oxidative stress in athleteis. The purpose of this study was to investigate the effects of curcumin supplementation and light resistance training on superoxide dismutase (SOD) activity of kidney tissues and malondialdehyde (MDA) as oxidative status level during 8 weeks of endurance training in male wistar rats.
Methodology: 33 Male Wistar rats (weight= 255.62±19.99 g, age= 8 weeks) were randomly divided in to 4 groups; Control (n=6), Endurance(n=9), Endurance+curcumin(n=9), Endurance+curcumin+resistance(n=9) after 1 week orentatioon period .Endurance training(8 weeks, 5 sessions per week) were carried out on an animal treadmill. The speed and duration of running were 10 m/min and 30 min at first week, respectively and reached to the 35 m/min and 70 min up to the last week. Resistance training (8 weeks, 2 sessions per week) were carried out on vertical ladder with extra weight (30-70% BW) fixed on tail. The animals received curcumin supplement by sub peritoneal injection (8 weeks, 3 sessions per week, 30 mg/kg.Bw). SOD activity and MDA levels of kidney tissues were measured by spectrophotometric. Analysis of variance was used to analysis of the data.
Results: The results of this study showed that strenuous endurance training decreases the SOD activity of and increases the MDA levels of kidney tissues (PConclusion: Based on findings of this study, it seems that curcumin supplementation and curcumin combined with light resistance training can prevent oxidative stress during strenuous endurance training.

کلیدواژه‌ها [English]

  • Endurance training
  • light resistance training
  • Curcumin supplementation
  • Antioxidant capacity
  • Tissue of kidney
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