تاثیر شدت‌های متوسط فعالیت هوازی حاد یک جلسه‌ای بر تعداد سلول‌های بنیادی +CD34 در گردش در مردان میانسال

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزشی، دانشکده علوم تربیتی و روانشناسی، دانشگاه محقق اردبیلی، ایران

2 استاد گروه فیزیولوژی ورزشی، دانشکده علوم تربیتی و روانشناسی، دانشگاه محقق اردبیلی، اردبیل، ایران

چکیده

سابقه و هدف : هدف از پژوهش حاضر بررسی اثر شدت­های متوسط یک وهله فعالیت هوازی حاد بر بسیج سلول­های بنیادیCD34+  در مردان میانسال بود.
مواد و روش: 24 مرد میانسال (با میانگین سنی 51/3 ± 21/51 سال، قد: 67/ 5± 71/174 سانتی­متر و وزن 94/8 ± 15/82 کیلوگرم) به­ عنوان نمونه­ی تحقیق انتخاب و به صورت تصادفی به 3 گروه 8 نفره (گروه کنترل، گروه تجربی الف و گروه تجربی ب) تقسیم شدند. گروه­های تجربی (الف:60% و ب: 70%  VO2max) به مدت 30 دقیقه روی نوارگردان دویدند. برای اندازه­گیری سلول­های بنیادی CD34+، قبل و 10 دقیقه بعد از فعالیت نمونه­های خونی جمع­آوری شد. برای مقایسه بین و درون­گروهی به ترتیب از آزمون­های تحلیل واریانس یک راهه و تی همبسته استفاده کردیم.
یافته­ ها: هر دو شدت فعالیت هوازی باعث افزایش معنی­دار در تعداد سلول­های CD34+ شد، در گروه الف این افزایش 14% ( 002/0P= ) بود، در حالی که این اثرگذاری در گروه ب با 22% افزایش بیشتر بود (003/0 P=)، ولی بین دو گروه تفاوت معنی­داری مشاهده نشد و گروه کنترل هیچ­گونه تغییر معنی­داری از پیش آزمون به پس آزمون (084/0 =P) نداشت. همچنین ارتباط بین تغییرات سطوح سلول­های CD34+ با نسبت LDL/HDL و Chol/HDL معنی­دار نبود.
نتیجه­ گیری: فعالیت بدنی با شدت­های 60 و 70 درصد حداکثر اکسیژن مصرفی می­تواند به طور معنی­داری موجب افزایش تعداد سلول­های CD34+ در گردش خون محیطی شوند و به سبب نقش بالقوه­ی این سلول­ها در آنژیوژنز و نئوواسکولوژنز احتمالا می­توانند به ترمیم و نوزایی عروق کمک کنند.
 

کلیدواژه‌ها


عنوان مقاله [English]

The effect of acute bout of aerobic activity with moderate intensities on CD34+ stem cells circulating numbers in middle-aged men

نویسندگان [English]

  • Elahe Mamashli 1
  • Marefat Siahkouhian 2
1 Faculty of Education and Psychology, Mohaghegh Ardabali University, Ardabil, Iran
2 Professor of Exercise Physiology,Faculty of Education and Psychology, Mohaghegh Ardabali University, Ardabil, Iran
چکیده [English]

Background&Purpose: The purpose of this study was to investigate the effect of acute bout  of aerobic activity with moderate intensities on CD34+ stem cells  circulating numbers in middle-aged men.
Methodology: 24 middle aged men (Age 51.21 ± 3.51 yrs, Height 174.71 ± 5.67 cm and Weight 82.15 ± 8.94 kg) selected as the samples in this study and randomly divided into 3 groups (control group N: 8, experimental group A N: 8, experimental group B N:8). experimental groups (A:60% and B:70% VO2max) ran for 30 minutes on the treadmill. To measuring CD34+ stem cells, befor and 10 minutes after the test protocol, blood samples were taken. To compare variation between and within the groups, we used One-Way analysis of variance test (ANOVA) and paired- samples T Test, respectively.
Results: Both intensity of aerobic activity significantly increased the number of CD34+ cells, in group A, this increase was 14% (P = 0.002), while this effect was higher in group B with 22% (P =0.003), but no significant difference was observed between the two groups and the control group no significant change from pre-test to post-test (P= 0.082). The relationship between changes in levels of CD34+ cells with LDL/HDL and Chol/HDL ratios was not significant.
Conclusion: aerobic activity with intensities 60% and 70% of Vo2max can significantly increased the number of circulating CD34+ cell in the peripheral blood and because of the potential role of these cells in neo-angiogenesis and vasculogenesis, they can possibly help repair and regeneration vessels.
 

کلیدواژه‌ها [English]

  • stem cells CD34+
  • Angiogenesis
  • neo vasculogenesis
  • aerobic activity intensity

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