نوع مقاله : مقاله پژوهشی
نویسندگان
1 کارشناس ارشد فیزولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه اصفهان، اصفهان، ایران
2 استاد گروه فیزولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه اصفهان، اصفهان، ایران
3 استادیار گروه فیزولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه اصفهان، اصفهان، ایران
4 استاد گروه فیزیولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی، اصفهان، ایران
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Background and Objective: Diabetes mellitus is associated with neurodegenerative alterations in the hippocampus, including reduced neurotrophic factors and impaired synaptic plasticity. The present study aimed to compare the effects of endurance training and high-intensity interval training (HIIT) on the gene and protein expression of brain-derived neurotrophic factor (BDNF) and tropomyosin receptor kinase B (TrkB) in the hippocampus of diabetic male Wistar rats.
Materials and Methods: In this experimental study, forty male Wistar rats were randomly assigned to four groups: control, sedentary diabetic, diabetic + endurance training, and diabetic + HIIT. Diabetes was induced by a single intraperitoneal injection of streptozotocin (55 mg/kg). The exercise groups completed an eight-week intervention consisting of moderate-intensity endurance training or HIIT. At the end of the intervention, hippocampal tissues were collected. The mRNA expression of BDNF and TrkB was quantified using quantitative real-time polymerase chain reaction (qRT-PCR), and the corresponding protein expression levels were evaluated by Western blot analysis.
Results: iabetes significantly reduced the gene and protein expression of BDNF and TrkB in the hippocampus compared with the control group (P ≤ 0.05). Both endurance training and high-intensity interval training (HIIT) significantly increased the mRNA expression of BDNF and TrkB compared with the sedentary diabetic group (P ≤ 0.05), with HIIT producing a greater increase in BDNF mRNA expression. Although protein expression of BDNF and TrkB showed an upward trend following both exercise interventions, these increases did not reach statistical significance.
Conclusion: Both endurance training and HIIT significantly improved the gene expression of BDNF and TrkB in the hippocampus of diabetic rats. Among the two exercise modalities, HIIT produced a greater increase in BDNF gene expression, suggesting a stronger capacity to modulate hippocampal neurotrophic signaling. These findings indicate that exercise training, particularly HIIT, may represent a promising non-pharmacological strategy for improving the molecular status of the hippocampus in diabetes. However, further studies are needed to determine whether these molecular changes translate into enhanced neuroplasticity and improved cognitive function.
کلیدواژهها [English]
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