بررسی تغییرات سطوح IGF-1 و PDGF سرمی زنان غیرفعال در اثر تمرینات ترکیبی سنتی و تمرینات ترکیبی با محدودیت جریان خون

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزشی، گروه تربیت‌بدنی و علوم ورزشی، واحد نیشابور، دانشگاه آزاد اسلامی، نیشابور، ایران

2 استادیار گروه تربیت بدنی و علوم ورزشی، واحد مشهد، دانشگاه آزاد اسلامی، مشهد، ایران

3 استادیار گروه تربیت بدنی و علوم ورزشی، واحد نیشابور، دانشگاه آزاد اسلامی، نیشابور، ایران

10.22080/jaep.2024.27146.2185

چکیده

اهداف: تمرینات مقاومتی برای افزایش حجم توده عضلانی توصیه می­شود. اخیراً تمرینات همراه با محدودیت جریان خون مورد توجه قرار گرفته است. بنابراین هدف از تحقیق حاضر بررسی اثر 12 هفته تمرین ورزشی ترکیبی همراه با محدودیت جریان خون بر سطح سرمی عامل رشد شبه انسولین-1 (IGF-1) و عامل رشد مشتق از پلاکت (PDGF) سرمی زنان جوان غیر فعال بود.
روش مطالعه: 45 زن غیرفعال به صورت تصادفی انتخاب و در سه گروه گروه تمرین ترکیبی همراه با BFR (15 نفر)، تمرین ترکیبی سنتی (TRT) (15 نفر) و گروه کنترل (15 نفر) تقسیم شدند. گروه BFR، تمرینات ترکیبی را با بستن کاف و شدت تمرین 20 درصد یک تکرار بیشینه انجام دادند؛ گروه تمرین TRT، تمرین مقاومتی با 80 درصد 1RM را 3 جلسه در هفته برای 12 هفته در روزهای متوالی انجام دادند. نمونه سرم قبل و بعد از 12 هفته تمرین از آزمودنی­ها گرفته شد. سطح سرمی IGF-1 و PDGF به روش الایزا اندازه­گیری شد. داده­ها بااستفاده از تی وابسته، آنالیز واریانس یک­طرفه و آزمون تعقیبی توکی در سطح معنا­داری 05/0>P تحلیل شدند.
یافته­ ها: سطح سرمی PDGF و IGF-1 در هر دو گروه تمرین به طور معناداری نسبت به پیش آزمون افزایش داشت (05/0>p). همچنین سطح سرمی PDGF و IGF-1 در گروه های BFR و TRT به طور معناداری بالاتر از گروه کنترل بود (001/0=p). سطح این شاخص­ها در گروه­های تمرینی تفاوت معناداری را نشان نداد.
نتیجه گیری: بنابراین به توجه به عدم تفاوت معنادار بین تمرین ترکیبی سنتی و تمرینات ترکیبی کم شدت همراه با BFR بنابراین می توان به زنان غیرفعال توصیه کرد که به جای تمرینات شدید از تمرینات کم شدت همراه با BFR با نتایج مشابه استفاده کنند.

کلیدواژه‌ها


عنوان مقاله [English]

Study of Changes in serum IGF-1 and PDGF levels in inactive women due to traditional combined training and combined training with blood flow restriction

نویسندگان [English]

  • Leila Jaafari 1
  • Mahmoud Soltani 2
  • Mohammad Reza Hosseinabadi 3
  • Amene Barjaste Yazdi 3
1 Department of Physical Education and Sport Sciences Neyshabur Branch, Islamic Azad University, Neyshabur, Iran
2 Department of Physical Education and Sport Sciences. Mashhad Branch, Islamic Azad University, Mashhad, Iran
3 Department of Physical Education and Sport Sciences. Neyshabur Branch, Islamic Azad University, Neyshabur, Iran.
چکیده [English]

Objectives: Resistance training is recommended for increasing muscle mass. Recently, blood flow restriction training has received attention. Therefore, the aim of the present study was to investigate the effect of 12 weeks of combined exercise training with blood flow restriction on serum levels of insulin-like growth factor-1 (IGF-1) and platelet-derived growth factor (PDGF) in inactive young women.
Methods: 45 inactive young women were selected and randomly divided into three groups: combined exercise group with BFR (n=15), traditional combined exercise (TRT) (n=15), and control group (n=15). The BFR group performed combined exercises with cuff closure and an exercise intensity of 20% of one repetition maximum; the TRT exercise group performed resistance training with 80% of 1RM 3 sessions per week for 12 weeks on consecutive days. Serum samples were collected from the subjects before and after 12 weeks of training. Serum levels of IGF-1 and PDGF were measured by ELISA. Data were analyzed using paired t-test, one-way analysis of variance, and Tukey's post hoc test at a significance level of P<0.05.
 Results: Serum levels of PDGF and IGF-1 in both exercise groups increased significantly compared to the pre-test (p<0.05). Also, serum levels of PDGF and IGF-1 in the BFR and TRT groups were significantly higher than the control group (p=0.001). The levels of these indicators in the exercise groups did not show a significant difference.
Conclusion: Therefore, considering the lack of significant difference between traditional combined exercise and low-intensity combined exercise with BFR, it can be recommended to inactive women to use low-intensity exercises with BFR instead of intense exercises with similar results.

کلیدواژه‌ها [English]

  • Combined Training
  • Blood Flow Restriction
  • PDGF
  • IGF-1
  • Inactive Women
##Ahtiainen, J. P., Pakarinen, A., Kraemer, W. J., & Häkkinen, K. (2003). Acute hormonal and neuromuscular responses and recovery to forced vs. maximum repetitions multiple resistance exercises. International journal of sports medicine, 24(06), 410-418. ##Cao, R., Bråkenhielm, E., Li, X., Pietras, K., Widenfalk, J., Östman, A., . . . Cao, Y. (2002). Angiogenesis stimulated by PDGF‐CC, a novel member in the PDGF family, involves activation of PDGFR‐aa and‐ap receptors. The FASEB Journal, 16(12), 1575-1583. ##Frontera, W. R., Hughes, V. A., Fielding, R. A., Fiatarone, M. A., Evans, W. J., & Roubenoff, R. (2000). Aging of skeletal muscle: a 12-yr longitudinal study. Journal of applied physiology, 88(4), 1321-1326. ##Gu, J.-W., Gadonski, G., Wang, J., Makey, I., & Adair, T. H. (2004). Exercise increases endostatin in circulation of healthy volunteers. BMC physiology, 4, 1-6. ##Gubbi, S., Quipildor, G. F., Barzilai, N., Huffman, D. M., & Milman, S. (2018). 40 YEARS of IGF1: IGF1: the Jekyll and Hyde of the aging brain. Journal of molecular endocrinology, 61(1), T171-T185. ##Hafstad, A. D., Lund, J., Hadler-Olsen, E., Höper, A. C., Larsen, T. S., & Aasum, E. (2013). High-and moderate-intensity training normalizes ventricular function and mechanoenergetics in mice with diet-induced obesity. Diabetes, 62(7), 2287-2294. ##Ieronimakis, N., Hays, A., Prasad, A., Janebodin, K., Duffield, J. S., & Reyes, M. (2016). PDGFRα signalling promotes fibrogenic responses in collagen‐producing cells in Duchenne muscular dystrophy. The Journal of pathology, 240(4), 410-424. ##Jensen, A. E., Palombo, L. J., Niederberger, B., Turcotte, L. P., & Kelly, K. R. (2016). Exercise training with blood flow restriction has little effect on muscular strength and does not change IGF-1 in fit military warfighters. Growth Hormone & IGF Research, 27, 33-40. ##Jiang, Q., Lou, K., Hou, L., Lu, Y., Sun, L., Tan, S. C., . . . Pang, S. (2020). The effect of resistance training on serum insulin-like growth factor 1 (IGF-1): a systematic review and meta-analysis. Complementary therapies in medicine, 50, 102360. ##Karabulut, M., Abe, T., Sato, Y., & Bemben, M. G. (2010). The effects of low-intensity resistance training with vascular restriction on leg muscle strength in older men. European journal of applied physiology, 108, 147-155. ##Kawada, S., & Ishii, N. (2005). Skeletal muscle hypertrophy after chronic restriction of venous blood flow in rats. Medicine & Science in Sports & Exercise, 37(7), 1144-1150. ##Ladlow, P., Coppack, R. J., Dharm-Datta, S., Conway, D., Sellon, E., Patterson, S. D., & Bennett, A. N. (2018). Low-load resistance training with blood flow restriction improves clinical outcomes in musculoskeletal rehabilitation: a single-blind randomized controlled trial. Frontiers in physiology, 9, 1269##.Lang, T., Streeper, T., Cawthon, P., Baldwin, K., Taaffe, D. R., & Harris, T. (2010). Sarcopenia: etiology, clinical consequences, intervention, and assessment. Osteoporosis international, 21, 543-559. ##Loenneke, J., Fahs, C., Wilson, J., & Bemben, M. (2001). Blood flow restriction: the metabolite/volume threshold theory. Medical hypotheses, 77(5), 748-752. ##Loenneke, J. P., & Pujol, T. J. (2009). The use of occlusion training to produce muscle hypertrophy. Strength & Conditioning Journal, 31(3), 77-84. ##Lopes, K. G., Bottino, D. A., Farinatti, P., de Souza, M. d. G. C., Maranhão, P. A., de Araujo, C. M. S., . . . de Oliveira, R. B. (2019). Strength training with blood flow restriction–a novel therapeutic approach for older adults with sarcopenia? A case report. Clinical interventions in aging, 1461-1469##.Manini, T. M., Yarrow, J. F., Buford, T. W., Clark, B. C., Conover, C. F., & Borst, S. E. (2012). Growth hormone responses to acute resistance exercise with vascular restriction in young and old men. Growth Hormone & IGF Research, 22(5), 167-172. ##Novak, S., Madunic, J., Shum, L., Vucetic, M., Wang, X., Tanigawa, H., . . . Kalajzic, I. (2023). PDGF inhibits BMP2-induced bone healing. npj Regenerative Medicine, 8(1), 3. ##Olfert, I. M., Howlett, R. A., Tang, K., Dalton, N. D., Gu, Y., Peterson, K. L., . . . Breen, E. C. (2009). Muscle‐specific VEGF deficiency greatly reduces exercise endurance in mice. The Journal of physiology, 587(8), 1755-1767. ##Olney, R. C. (2003). Regulation of bone mass by growth hormone. Medical and pediatric oncology, 41(3), 228-234. ##Piñol-Jurado, P., Gallardo, E., de Luna, N., Suárez-Calvet, X., Sánchez-Riera, C., Fernández-Simón, E., . . . Díaz-Manera, J. (2017). Platelet-derived growth factor BB influences muscle regeneration in Duchenne muscle dystrophy. The American journal of pathology, 187(8), 1814-1827. ##Pullinen, T., Mero, A., Huttunen, P., Pakarinen, A., & Komi, P. V. (2002). Resistance exercise-induced hormonal responses in men, women, and pubescent boys. Medicine & Science in Sports & Exercise, 34(5), 806-813. ##Rezaee, Z., Javaheri, S. A. A. H., Rashidlamir, A., Oral, O., & Stavropoulos, E. (2022). Effects of BFR training in serum NRG-1 and IL-6 in overweight postmenopausal women. Scientific Chronicles, 27(4): 541-554##Saraf, A., Goyal, M., & Goyal, K. (2024). Historical Perspective and Introduction to Blood Flow Restriction Training Blood Flow Restriction Training (pp. 23-40): Jenny Stanford Publishing. ##Sarikhani, N., Gholipour, M., & Malekipooya, M. (2022). Effect of exercise activity with different intensity and volume on the expression of PDGF-B and FGF-2 genes in subcutaneous adipose tissue of Wistar rats. medical journal of mashhad university of medical sciences, 65(3), 1259-1267. ##Schoenfeld, B. J. (2013). Postexercise hypertrophic adaptations: a reexamination of the hormone hypothesis and its applicability to resistance training program design. The Journal of Strength & Conditioning Research, 27(6), 1720-1730. ##Seo, D. I., Jun, T. W., Park, K. S., Chang, H., So, W. Y., & Song, W. (2010). 12 weeks of combined exercise is better than aerobic exercise for increasing growth hormone in middle-aged women. International journal of sport nutrition and exercise metabolism, 20(1), 21–26. ##Seyedi, S. H., Khajei, R., Rashidlamir, A., Ramezanpour, M. R., & Mehrzad, J. (2021).  Effect of eight weeks of aerobic-resistance training on stromal cell-derived factor-1 and platelet-derived growth factor level in patients with coronary artery bypass grafting: A clinical trial study. Journal of Gorgan University of Medical Sciences, 23(2), 16-23. ##Sundberg, C. J. (1994). Exercise and training during graded leg ischaemia in healthy man with special reference to effects on skeletal muscle. Acta physiologica Scandinavica. Supplementum, 615, 1-50. ##Thankamony, A., Capalbo, D., Marcovecchio, M. L., Sleigh, A., Jørgensen, S. W., Hill, N. R., . . . Juul, A. (2014). Low circulating levels of IGF-1 in healthy adults are associated with reduced β-cell function, increased intramyocellular lipid, and enhanced fat utilization during fasting. The Journal of Clinical Endocrinology & Metabolism, 99(6), 2198-2207. ##Trenerry, M. K., Della Gatta, P. A., Larsen, A. E., Garnham, A. P., & Cameron‐Smith, D. (2011). Impact of resistance exercise training on interleukin‐6 and JAK/STAT in young men. Muscle & nerve, 43(3), 385-392. ##Velloso, C. (2008). Regulation of muscle mass by growth hormone and IGF‐I. British journal of pharmacology, 154(3), 557-568. ##Yang, X., Kord-Varkaneh, H., Talaei, S., Clark, C. C., Zanghelini, F., Tan, S. C., . . . Zhang, Y. (2020). The influence of metformin on IGF-1 levels in humans: A systematic review and meta-analysis. Pharmacological research, 151, 104588. ##Yüksel, B., Özbek, M. N., Mungan, N. Ö., Darendeliler, F., Budan, B., Bideci, A., . . . Yeşilkaya, E. (2011). Serum IGF-1 and IGFBP-3 levels in healthy children between 0 and 6 years of age. Journal of clinical research in pediatric endocrinology, 3(2), 84. ##