اثر 10 هفته تمرین هوازی پس از پیوند سلول های بنیادی مزانشیمی بر مقادیر آپلین 13 و برون ده قلبی در موش‌های صحرایی مدل انفارکتوس قلبی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری گروه فیزیولوژی ورزشی، دانشکده علوم انسانی، واحد ساری، دانشگاه آزاد اسلامی، ساری، ایران.

2 استادیار گروه فیزیولوژی ورزشی، دانشکده علوم انسانی، واحد ساری، دانشگاه آزاد اسلامی، ساری، ایران

3 استادیار گروه فیزیولوژی ورزشی، دانشکده علوم انسانی، واحد ساری، دانشگاه آزاد اسلامی، ساری، ایران.

10.22080/jaep.2024.26525.2170

چکیده

اهداف: امروزه به استفاده از سلول­های بنیادی مزانشیمی و اثرات مثبت تمرینات ورزشی در درمان بیماری­های مختلف از جمله انفارکتوس قلبی توجه شده است، بنابراین هدف از اجرای این پژوهش بررسی اثر 10 هفته تمرین هوازی پس از پیوند سلول­های بنیادی مزانشیمی بر مقادیر آپلین13 و برونده قلبی موش­های صحرایی مدل انفارکتوس قلبی بود.
روش مطالعه: در این مطالعه تجربی از 60 سر موش صحرایی نر استفاده شد که به 6 گروه 10 تایی تقسیم شدند. گروه کنترل سالم، کنترل بیمار، شم، سلول، تمرین و سلول + تمرین. برای ایجاد انفارکتوس قلبی، شریان پایین رونده قدامی با استفاده از نخ سیلک 6/0 به طور کامل مسدود شد. سلول­های بنیادی پس از استخراج، با استفاده از لام نئوبار، حدود 106 سلول برای هر موش از طریق سیاهرگ دمی تزریق شد. موش­های گروه تمرینی به مدت 10 هفته تمرین هوازی را با شدت 17 متر بر دقیقه انجام دادند. اندازه­گیری برون ده قلبی به وسیله دستگاه اکوکاردیوگرافی و مقادیر آپلین 13 به وسیله کیت آزمایشگاهی انجام شد.
یافته­ها: یافته­ها نشان داد که القاء انفارکتوس قلبی موجب کاهش معنادار سطوح اپلین 13و برون ده قلبی در گروه بیمارنسبت به گروه کنترل سالم شد (05/0>p). تمرین هوازی و سلول­های بنیادی موجب افزایش معنادار سطح آپلین 13 و برون ده قلبی در گروه­های تجربی (تمرین و سلول­های بنیادی) در مقایسه با گروه کنترل (بیمار) شد (05/0p<).
نتیجه گیری: نتایج حاصل از این پژوهش نشان داد که 10 هفته تمرین هوازی پس از پیوند سلول­های بنیادی مزانشیمی احتمال دارد از طریق افزایش Apelin-13 عملکرد انقباضی را بهبود بخشد و موجب افزایش برون ده قلبی شود.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of 10 Weeks of Aerobic Training After Mesenchymal Stem Cells Transplantation on Apelin 13 Levels and Cardiac Output in Heart Infarction Model Rats

نویسندگان [English]

  • Fatemeh Kolbadinezhad 1
  • Seyed Abdollah Hashemvarzi 2
  • Abdolreza Jafari Chashmi 3
1 Exercise Physiology Department, Faculty of Humanities, Sari Branch, Islamic Azad University, Sari, Iran.
2 Exercise Physiology Department, Faculty of Humanities, Sari Branch, Islamic Azad University, Sari, Iran.
3 Exercise Physiology Department, Faculty of Humanities, Sari Branch, Islamic Azad University, Sari, Iran.
چکیده [English]

Objectives: The use of mesenchymal stem cells and the positive effects of exersise training have been noticed today in the treatment of various diseases, including heart infarction. The purpose of this research was to investigate the effect of 10 weeks of aerobic training after mesenchymal stem cell transplantation on the Apelin 13 and cardiac output of heart infarction model rats.
Methods: In this experimental study, 60 male rats were used and divided into 6 groups of 10 each, healthy control group, patient control, sham, cell, exercise and cell+exercise. To cause cardiac infarction, the anterior descending artery was completely blocked using 0.6 silk thread. After extracting stem cells, using Neobar slide, about 106 cells were injected for each mouse through the tail vein immediately after induction of cardiac infarction. Rats in the training group performed aerobic training with an intensity of 17 meters per minute for 10 weeks. Cardiac output and Apelin level 13 were measured.
Results: The findings showed that the induction of cardiac infarction caused a significant decrease in the levels of apelin 13 and cardiac output in the patient group compared to the healthy control group (p<0.05). Aerobic training and stem cells significantly increased the level of apelin 13 and cardiac output in experimental groups (exercise and stem cells) compared to the control group (patient) (p<0.05).
Conclusion: The results of this research showed that 10 weeks of aerobic training after mesenchymal stem cell transplantation may improve contractile function and increase cardiac output through the increase of Apelin-13.

کلیدواژه‌ها [English]

  • Aerobic Training
  • Mesenchymal Stem Cell
  • Apelin 13
  • Cardiac Output
  • Cardiac Infarction
 
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