بیان پروتئین های PPARα و PGC1α در بافت قلبی موشهای صحرایی مبتلا به دیابت نوع 2 متعاقب تمرینات تناوبی با شدت بالا

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه شهید چمران اهواز، اهواز، ایران.

2 استادیار گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه شهید چمران اهواز، اهواز، ایران

3 استاد گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه شهید چمران اهواز، اهواز، ایران

10.22080/jaep.2023.23937.2113

چکیده

اهداف: فعالیت بدنی در بیماران مبتلا به دیابت می‌تواند ساختار و عملکرد میوکارد را تحت تأثیر قرار دهد، لذا هدف از مطالعه حاضر بررسی اثر ۸ هفته تمرین تناوبی با شدت بالا (HIIT) بر بیان پروتئین­های PPARα و PGC1α در بافت قلب موش‌های صحرایی نر نژاد ویستار مبتلا به دیابت نوع ۲ بود.
روش مطالعه: در پژوهش حاضر 28 سر موش صحرایی نر نژاد ویستار ۸ هفته‌ای به صورت تصادفی به 4 گروه سالم کنترل، سالم تمرین، دیابت کنترل و دیابت تمرین تقسیم شدند. پس از یک دوره رژیم غذایی پرچرب و القاء دیابت، موش‌های صحرایی در گروه‌های سالم تمرین و دیابت تمرین پروتکل تمرینی HIIT را به مدت  ۸ هفته و ۵ جلسه در هفته انجام دادند. ۴۸ ساعت پس از آخرین جلسه تمرینی بافت قلب خارج شد و با استفاده از روش وسترن بلات سطوح پروتئین‌های PGC-1α و PPARα بررسی شد. سپس داده‌ها با استفاده از آزمون آنوای یک راهه و آزمون تعقیبی توکی در سطح معناداری 05/0P< مورد بررسی قرار گرفت.
یافته ­ها: نتایج پژوهش نشان داد سطوح بیان پروتئین‌ PGC-1α در گروه‌ دیابت کنترل نسبت به گروه سالم کنترل کاهش معناداری پیدا کرد (001/0P=) و در گروه دیابت تمرین نسبت به گروه دیابت کنترل به صورت معناداری افزایش یافت (001/0P=). همچنین، سطوح بیان پروتئین PPARα در گروه‌های دیابت کنترل و دیابت تمرین نسبت به گروه‌های سالم به صورت معناداری افزایش یافت (001/0P=). و مقدار پروتئین PPARα در گروه دیابت تمرین نسبت به گروه دیابت کنترل کاهش معناداری نشان داد (001/0P=).
نتیجه گیری: تمرینات HIIT احتمالاً می‌تواند نقش موثری در تنظیم مسیر سیگنالی PPARα/PGC1α داشته و تغییرات نامطلوب ناشی از بیماری دیابت را در این مسیر سیگنالیِ مرتبط با بیوژنز میتوکندریایی تعدیل کند.

کلیدواژه‌ها


عنوان مقاله [English]

Expression of PPARα and PGC1α proteins in cardiac tissue of male rats with type 2 diabetes after high intensity interval training

نویسندگان [English]

  • Mozhgan Dastyar 1
  • Mohammad Rami 2
  • Abdolhamid Habibi 3
1 Department of Exercise Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
2 Department of Exercise Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3 Department of Exercise Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
چکیده [English]

Objectives: Physical activity in patients with diabetes can affect myocardial structure and function, the aim of this study was to evaluate the effect of 8 weeks of high intensity interval training (HIIT) on expression of PPARα and PGC1α proteins in the heart tissue of male Wistar rats with type 2 diabetes.
Methods: In the present study, 28 male Wistar rats were randomly divided into four groups Control Healthy, Exercise Healthy, Diabetes Control, and Diabetes Exercise. After a period of high-fat diet and then after induction of diabetes, animals in Diabetes Exercise and Exercise Healthy groups performed the HIIT training protocol for 8 weeks and 5 sessions per week. 48 hours after the last training session, cardiac tissue was extracted and the levels of PGC-1α and PPARα proteins were assessed using Western blotting. Data were analyzed using one-way ANOVA and Tukey post hoc test at a significance level of P<0.05.
Results: The results showed that the expression levels of PGC-1α protein in the Diabetes Control group decreased significantly compared to the Control Healthy group (P=0.001) and increased significantly in the Diabetes Exercise group compared to the Diabetes Control group (P=0.001). Also, expression levels of PPARα protein in the Diabetes Control and Diabetes Exercise groups increased significantly compared to the healthy groups (P=0.001). Moreover, the amount of PPARα protein showed a significant decrease in the Diabetes Exercise group compared to the Diabetes Control group (P=0.001).
Conclusion: HIIT exercises can probably play an effective role in regulating the PPARα/PGC1α signaling pathway and modulate the adverse changes caused by diabetes in this signaling pathway related to mitochondrial biogenesis.

کلیدواژه‌ها [English]

  • Diabetes
  • HIIT
  • Mitochondria
  • Cardiac
  • Cardiomyopathy
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