تاثیر تمرین استقامتی بر متابولیسم لیپیدها در کبد موش‌های پیش‌دیابت

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه اصفهان، اصفهان، ایران

2 گروه زیست فناوری سلولی، مرکز تحقیقات علوم سلولی، پژوهشکده زیست فناوری جهاد دانشگاهی، پژوهشگاه رویان، اصفهان

10.22080/jaep.2023.24794.2125

چکیده

اهداف: پیش­ دیابت عامل خطر در دیابت است و در نتیجه سبک زندگی سالم برای جلوگیری از پیش­ دیابت مهم است. این مطالعه با هدف تعیین اثر تمرین استقامتی بر متابولیسم لیپیدهای کبدی در موش‌های نر C57BL/6 پیش دیابتی انجام شد.
روش مطالعه: 21 موش به طور تصادفی به دو گروه تقسیم شدند. یک گروه به مدت 12 هفته با رژیم غذایی معمولی  (7=n , ND)، و دیگری رژیم غذایی پرچرب (14=n , HFD) شدند. پس از 12 هفته نگهداری اولیه موش‌های HFD  آزمون تحمل گلوکز انجام دادند و بعد از ابتلا به پیش­ دیابت به دو گروه تمرین(EX) ،  و پیش­دیابت (preD) تقسیم شدند. گروه تمرینی به مدت 45 دقیقه روزانه، 5 روز در هفته به مدت 10 هفته تمرین استقامتی با تردمیل انجام دادند. 24 ساعت پیش از قربانی نمودن نمونه ­ها وزن­ گیری و آزمون مجدد تحمل گلوکز برای ثبت وخامت یا میزان بهبود انجام و پس از آن پلاسما و کبد آزمودنی­ها جمع­ آوری شد.. از مقادیر استیل کوآکربوکسیلاز بتا (ACC2)، کارنیتین پالمیتول ترانسفراز یک (CPT1) و گیرنده پروکسی­زوم آلفا برای ارزیابی بتا اکسیداسیون استفاده شد. تفسیر داده ­ها با استفاده از آزمون تحلیل واریانس یک­طرفه با سطح معناداری p<0.05 انجام شد.
یافته­ ها: در گروه پیش ­دیابت وزن ، گلوکز، انسولین، مقاومت انسولینی و پروفایل لیپیدی افزایش معناداری به نسبت گروه نرمال تجربه کرد. در گروه‌ EX فاکتورهای مذکور با کاهش معنادار همراه بود (p<0.01). غلظت پروتئین CPT1، به عنوان فاکتور کلیدی بتااکسیداسیون، در گروه preD کاهش یافت و ACC2 که مهار کننده آن می­باشد افزایش داشت. تمرین استقامتی با معکوس کردن روند بیان این فاکتورها بهبود متابولیسم اسیدهای چرب را در کبد به دنبال داشت.
نتیجه گیری: به طور کلی preD تغییرات نامطلوب مرتبط با β اکسیداسیون را در کبد ایجاد کرد. درحالی که به نظر می­رسد تمرینات ورزشی این تغییرات را بهبود بخشید. این یافته ­ها نشان می دهد که احتمالا ورزش منظم یک رویکرد غیرداروئی موثر برای پیش ­دیابت است.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of endurance training on lipid metabolism in liver of prediabetic mice

نویسندگان [English]

  • Samaneh Shirkhani 1
  • Sayyed Mohammd Marandi 1
  • Milad Abdollahi 1
  • Fatemeh Janghorban 1
  • Zahra Ghavi 1
  • Zahra Safayinejad 2
  • Mohammad Hossein Nasr-Esfahani 2
1 Department of Sports Physiology, Faculty of Sports Sciences, University of Isfahan, Isfahan, Iran.
2 Department of Animal Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
چکیده [English]

Objectives: Prediabetes is a risk factor in diabetes and therefore a healthy lifestyle is important to prevent pre-diabetes. This study aimed to determine the effect of endurance training on hepatic lipid metabolism in prediabetic C57BL/6 male mice.
Methods: 21 mice were randomly divided into two groups. One was fed a normal diet (n=7, ND), and the other was fed a high-fat diet (n=14, HFD) for 12 weeks. After 12 weeks of initial maintenance, HFD s were subjected to a glucose tolerance test (GTT) and they were divided into two groups: exercise (EX) and pre-diabetes (preD). The training group trained for 45 minutes, 5 days a week, for 10 weeks. 24 hours before sacrifice, weighing and GTT were performed, and then the plasma and liver were obtained. The values of acetyl cocarboxylase beta (ACC2), carnitine palmitol transferase 1 (CPT1) and peroxisome receptor alpha were used to evaluate beta oxidation. Data interpretation was done using one-way analysis of variance with a significance level of p<0.05.
Results: In preD, weight, glucose, insulin, insulin resistance, and lipid profile experienced a significant increase compared to ND. In the EX, the mentioned factors were associated with a significant decrease (p < 0.01). CPT1 protein concentration, as a key factor of β-oxidation, decreased in preD the group, and ACC2, which is its inhibitor, increased. Endurance training improved fatty acid metabolism in the liver by reversing the expression process of these factors.
Conclusion: Generally, preD caused changes associated with β-oxidation in the liver, while it seems that endurance training improved all. These findings show that regular exercise is probably an effective nonpharmacological approach for pre-diabetes.

کلیدواژه‌ها [English]

  • Prediabetes
  • Liver
  • Lipogenesis
  • β-oxidation
  • Exercise
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