تاثیر شش هفته تمرین تناوبی خیلی شدید بربیان P53 و SRF در بافت تومور موش های ماده مبتلا به سرطان پستان

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه تهران پردیس بین المللی کیش، کیش، ایران

2 استادیارگروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه تهران پردیس بین المللی کیش، کیش، ایران.

3 استاد گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران

10.22080/jaep.2021.20809.2016

چکیده

سابقه و هدف: سرطان پستان، یکی از دلایل مرگ و میر زنان است. آنژیوژنز نقش مهمی در توسعه و متاستاز تومور دارد. اثر تمرین­های ورزشی بر مهار یا کاهش فرایند آنژیوژنز در بافت تومور به­طور کامل روشن نیست. لذا هدف پژوهش حاضر، بررسی آثارکمک درمانی تمرین تناوبی خیلی شدید بر بیان عوامل آنژیوژنزی  P53و  SRFبافت تومور سرطان پستان در موش­های آزمایشگاهی بود.
مواد و روشها: 16 سر موش بالبc  به­طور تصادفی به دو گروه کنترل (sedentary-tumor) و تمرین تناوبی خیلی شدید- تومور (HIIT-Tumor) تقسیم شدند. بعد از آشنایی با محیط آزمایشگاه، سلول­های سرطانی، زیر جلدی به موش­ها تزریق شد. دو هفته بعد، از گروه تمرین آزمون ظرفیت ورزشی انجام و سپس، شش هفته، سه جلسه در هفته، با تناوب 2 دقیقه دویدن 2 دقیقه استراحت پروتکل انجام شد. 48ساعت بعداز آخرین جلسه تمرین، موش­ها بی­هوش و قربانی و بافت تومور جدا شد. تجزیه و تحلیل بیان ژن­ها با نرم افزار جنکس نسخه 1/6  انجام شد (0/01≥P).
یافته‌ها: پژوهش حاضر نشان داد، تمرین HIIT موجب کاهش 27/5 برابری SRF و افزایش 97/5 برابری P53 در گروه تمرین نسبت به گروه  کنترل شد.
نتیجه گیری: به نظر می­رسد تمرین HIIT از راه­کاهش SRF و افزایش P53 می­تواند باعث سرکوب تومور و احتمالا"مانع از پیشرفت سرطان شود.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of six weeks of high intense intermittent training on the expression of P53 and SRF angiogenesis factors in tumor tissue of female mice with breast cancer

نویسندگان [English]

  • Tooran Soleimani 1
  • Reza Nuri 2
  • Abbasali Gaeini 3
1 Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran Kish International Campus, Kish, Iran
2 Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran Kish International Campus, Kish, Iran
3 Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences,University of Tehran,Tehran, Iran
چکیده [English]

Background and Purpose: Breast cancer causes death in women. Angiogenesis plays role in tumor development and metastasis. The effect of exercise on tumor tissue angiogenesis is not fully understood. Therefore, the aim of this study was to investigate the therapeutic effects of high intense intermittent exercise on the expression of angiogenesis factors, in breast cancer tumor tissue of laboratory mice.
Methodology: Sixteen C-balb mice were randomly divided into two groups of control (Sedentary-tumor) and high intense intermittent training-tumor (HIIT-Tumor). After getting acquainted with the laboratory environment, mice were hypodermically infused by tumor cells. After two weeks, exercise capacity test was performed on training group. Then, a six weeks exercise protocol, three sessions per week, with two minutes running and two minutes of rest, was performed. 48 hours after the last training session, the mice were anesthetized and the tumor tissues were isolated. Gene expression was analysed by Genex Software, version 6.1 (P≥0/01).
Results: This study showed that HIIT training decreased SRF by 5.27 times and increased P53 by 5.97 times in the training group, compared to the control group.
Conclusion: It seems that HIIT training, by decreasing SRF and increasing P53 can suppress the tumor and possibly prevent cancer progression.

کلیدواژه‌ها [English]

  • Breast cancer
  • Angiogenesis
  • SRF
  • P53
  • HIIT
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