اثرات تمرین شنا بر فاکتورهای التهابی و استرس اکسیداتیو کلیه‌ها‌ در موش‌های صحرایی ماده دیابتی و اواریکتومی‌شده

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکترای فارماکولوژی، واحد علوم و تحقیقات تهران، دانشگاه آزاد اسلامی، تهران، ایران

2 استادیار فیزیولوژی، دانشکده علوم دامپزشکی، واحد شبستر، دانشگاه آزاد اسلامی، شبستر، ایران.

3 استادیار فارماکولوژی، دانشکده علوم دامپزشکی، واحد شبستر، دانشگاه آزاد اسلامی، شبستر، ایران

چکیده

سابقه و هدف: یائسگی یک فرآیند فیزیولوژیک و دیابت یک بیماری متابولیک است که در زنان میانسال با شیوع بالایی اتفاق می‌افتد. در این مطالعه اثرات تمرین شنا بر برخی از شاخص­های بیوشیمیایی و فاکتورهای نکروز توموری آلفا (TNF-α) و اینترلوکین6 ((IL-6 بافت کلیه در موش‌های صحرایی اواریکتومی­شده و مبتلا به دیابت مورد بررسی قرار گرفت.
مواد و روشها: در این مطالعه تجربی 50 سر موش صحرایی ماده بالغ به پنج گروه 10تایی تقسیم و به مدت هشت هفته بررسی شدند. گروه اول شاهد، گروه دوم اواریکتومی­شده (دوطرفه)، گروه سوم موش­های اورایکتومی­ دیابتی و گروه چهارم اواریکتومی­ با تمرین شنا و گروه پنجم موش­های اواریکتومی دیابتی با تمرین شنا بودند. در انتهای دوره آزمایش نمونه­برداری­های بافتی به انجام رسید و تغییرات فاکتورهای بیوشیمیایی و پیش­التهابی بافت کلیه­ها تعیین شدند.
یافته­ها: غلظت گلوکز خون در موش‌های صحرایی دیابتی و اواریکتومی دیابتی افزایش داشت (001/0p <). غلظت­ مالون­دی­آلدئید در گروه­های اواریکتومی (025/0P=) و اواریکتومی دیابتی (001/0p <) افزایش و با تمرین کاهش یافت (040/0P=). فعالیت آنزیم­های سوپراکسیددیسموتاز (021/0P=)، گلوتاتیون­پراکسیداز (001/0p <) و کاتالاز (010/0P=) در گروه اواریکتومی­ دیابتی کاهش داشت که با اعمال تمرین، غلظت­ این آنزیم­ها افزایش یافتند (به ترتیب 023/0P=، 041/0P= و 027/0P=). سطوح فاکتورهای پیش­التهابی (TNF-α وIL-6) در گروه­های دیابتی و و اواریکتومی دیابتی افزایش نشان داد (001/0>P) در حالی­که در گروه تمرین، مقدار  این عوامل کاهش یافته بود.
نتیجه‌گیری: یائسگی و بیماری دیابت سبب افزایش فاکتورهای پیش­التهابی و تغییرات مضر در آنزیم­های آنتی­اکسیدانی کلیوی می­شوند. انجام تمرینات هوازی مانند شنا می­تواند به عنوان یک عامل موثر بر بهبود این شاخص­ها به انجام برسد.

کلیدواژه‌ها


عنوان مقاله [English]

Effects of Swim Training on Inflammatory Factors and Oxidative Stress of Kidneys in Diabetic and Ovariectomized Female Rats

نویسندگان [English]

  • Nima Alyari 1
  • Tohid Vahdatpour 2
  • Amirreza Karami-bonari 3
1 Department of Basic Science and Health, Faculty of Veterinary Sciences, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Department of Animal Sciences, Faculty of Animal Sciences and Veterinary Medicine, Shabestar Branch, Islamic Azad University, Shabestar, Iran
3 Department of Veterinary Medicine, Faculty of Animal Sciences and Veterinary Medicine, Shabestar Branch, Islamic Azad University, Shabestar, Iran.
چکیده [English]

Background and Purpose: Menopause is a physiologic process and diabetes is a metabolic disease that occurs with high prevalence in old women. In this study effects of exercise training on some biochemical parameters and factors ofTumor necrosis factor alpha (TNF-α) andInterleukin 6(IL-6) investigated in ovariectomized and diabetic rats.
Methodology: In this empirical study 50 mature female rats divided into five groups that 10 rats attributed to each group and were studied for eight weeks. The first as control group, the second group as ovariectomized (bilateral), third group as diabetic ovariectomized rats, fourth group as ovariectomized under swimming training, and fifth group as diabetic ovariectomized under swimming training rats. At the end experimental period, tissue sampling was performed and changes of biochemical and pre-inflammatory factors were determined.
Results: The blood glucose concentration increased in diabetic and diabetic ovariectomized (p < 0.001). The concentration of malondialdehyde increased in ovariectomized (P=0.025) and diabetic ovariectomized (p < 0.001) groups and decreased with training (P=0.040). The activity of superoxide dismutase (P=0.021), glutathione peroxidase (P <0.001) and catalase (P=0.010) decreased in diabetic ovariectomized group, which increased with training. (P=0.023, P=0.041 and P=0.027, respectively). The pre inflammatory factors (TNF-α and IL-6) were increased in diabetic and diabetic ovariectomized groups (p < 0.001) while training decreased the increased amount of these factors.
Conclusion: The menopause and diabetes lead to increasing of the renal pre inflammatory factors and harmful changing in the antioxidation enzymes. The aerobic training like as swimming can be used as an effective factor in improving these factors.

کلیدواژه‌ها [English]

  • Menopause
  • Diabetes
  • Kidney
  • Swimming
  • Oxidative Stress
##Elfattah LI. A comparative study between the effects of dietary soya and estrogen replacement therapy on the lung of ovariectomized albino rats: histological and immunohistochemical study. Egyptian journal of histology 2012; 35:34-42.##Filardo EJ, Quinn JA, Bland KI, Frackelton AR Jr. Estrogen-induced activation of Erk-1 and Erk-2 requires the G protein-coupled receptor homolog, GPR30, and occurs via trans-activation of the epidermal growth factor receptor through release of HB-EGF. Mol Endocrinol 2000; 14:1649-60.##Beardsmore CS, Godfrey S, Silverman M. Forced expiratory flow-volume curves in infants and young children. Eur Respir J Suppl 1989; 4:154S-159S.##García-Alvarez J, Ramirez R, Sampieri CL, Nuttall RK, Edwards DR, Selman M, et al. Membrane type-matrix metalloproteinase in idiopathic pulmonary fibrosis. Sarcoidosis Vasc Diffuse Lung Dis 2006; 23:13-21.##Vainshelboim B, Oliveira J, Yehoshua L, Weiss I, Fox BD, Fruchter O, et al. Exercise training-based pulmonary rehabilitation program is clinically beneficial for idiopathic pulmonary fibrosis. Respiration 2014; 88:378-88.##Cheng SM, Ho TJ, Yang AL, Chen IJ, Kao CL, Wu FN, et al. Exercise training enhances cardiac IGFI-R/PI3K/Akt and Bcl-2 family associated pro-survival pathways in streptozotocin-induced diabetic rats. Int J Cardiol 2013; 167:478-85.##Fernandez-Valverde SL, Taft RJ, Mattick JS. MicroRNAs in β-cell biology, insulin resistance, diabetes and its complications. Diabetes 2011; 60:1825-31.##Wang DT, Yin Y, Yang YJ, Lv PJ, Shi Y, Lu L, et al. Resveratrol prevents TNF-α-induced muscle atrophy via regulation of Akt/mTOR/FoxO1 signaling in C2C12 myotubes. Int Immunopharmacol 2014; 19:206-13.##Srinivasan K, Viswanad B, Asrat L, Kaul CL, Ramarao P. Combination of high-fat diet-fed and low-dose streptozotocin-treated rat: a model for type 2 diabetes and pharmacological screening. Pharmacol Res 2005; 52:313-20.##Somi MH, Hajipour B, Asl NA, Estakhri R, Azar AN, Zade MN, et al. Pioglitazone attenuates ischemia/reperfusion-induced liver injury in rats. Transplant Proc 2009; 41:4105-9.##Delmas-Beauvieux MC, Peuchant E, Dumon MF, Receveur MC, Le Bras M, Clerc M. Relationship between red blood cell antioxidant enzymatic system status and lipoperoxidation during the acute phase of malaria. Clin Biochem 1995; 28:163-9.##Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967; 70:158-69.##Kaczor, J., Hall, J., Payne, E., Tarnapolsky, M. Lowe intensity training decrease markers of oxidative stress in skeletal muscle of mdx mice. Free radical biology and medicine 2007; 43:145-154.##Pirola L, Johnston AM, Van Obberghen E. Modulation of insulin action. Diabetologia 2004; 47:170-84.##Davignon J, Jacob RF, Mason RP. The antioxidant effects of statins. Coron Artery Dis 2004; 15:251-8.##Wright DC, Geiger PC, Holloszy JO, Han DH. Contraction- and hypoxia-stimulated glucose transport is mediated by a Ca2+-dependent mechanism in slow-twitch rat soleus muscle. Am J Physiol Endocrinol Metab 2005; 288:E1062-6.##Praet SF, van Loon LJ. Exercise therapy in type 2 diabetes. Acta Diabetol 2009; 46:263-78.##Yoshihara R, Utsunomiya K, Gojo A, Ishizawa S, Kanazawa Y, Matoba K, et al. Association of polymorphism of estrogen receptor-alpha gene with circulating levels of adiponectin in postmenopausal women with type 2 diabetes. J Atheroscler Thromb 2009; 16:250-5.##El-Nasr AS, Diab FMA, Bahgat NM, Ahmed MA, Thabet SS, El-Dakkak SMY. Metabolic Effects of Estrogen and / or Insulin in Ovariectomized Experimentally Diabetic Rats. J American Science 2011; 7:432-444.##Carneiro FS, Webb RC, Tostes RC. Emerging role for TNF-α in erectile dysfunction. J Sex Med 2010; 7:3823-34.##Faustman D, Davis M. TNF receptor 2 pathway: drug target for autoimmune diseases. Nat Rev Drug Discov 2010; 9:482-93.##Romualdo PC, Lucisano MP, Paula-Silva FWG, Leoni GB, Sousa-Neto MD, Silva RAB, et al. Ovariectomy Exacerbates Apical Periodontitis in Rats with an Increase in Expression of Proinflammatory Cytokines and Matrix Metalloproteinases. J Endod 2018; 44:780-785.##Neto NI, Rodrigues ME, Hachul AC, Moreno MF, Boldarine VT, Ribeiro EB, et al. Hyperlipidic Diet Combined with Short-Term Ovariectomy Increases Adiposity and Hyperleptinemia and Decreases Cytokine Content in Mesenteric Adipose Tissue. Mediators Inflamm 2015; 2015:923248.##Van Horssen R, Ten Hagen TL, Eggermont AM. TNF-alpha in cancer treatment: molecular insights, antitumor effects, and clinical utility. Oncologist 2006; 11:397-408.##Chung MT, Lee YM, Shen HH, Cheng PY, Huang YC, Lin YJ, et al. Activation of autophagy is involved in the protective effect of 17β-oestradiol on endotoxaemia-induced multiple organ dysfunction in ovariectomized rats. J Cell Mol Med 2017; 21:3705-3717.##Sanches IC, Buzin M, Conti FF, Dias DDS, Dos Santos CP, Sirvente R, et al. Combined aerobic and resistance exercise training attenuates cardiac dysfunctions in a model of diabetes and menopause. PLOS One 2018; 13:e0202731.##Goyal R, Faizy AF, Siddiqui SS, Singhai M. Evaluation of TNF-α and IL-6 Levels in Obese and Non-obese Diabetics: Pre- and Postinsulin Effects. N Am J Med Sci 2012; 4:180-4.##