تاثیر تمرین مقاومتی بر غلظت پلاسمایی لیپوکالین-2، تعداد سلول‌های سفید خون و شاخص مقاومت به انسولین در مردان مبتلا به دیابت نوع 2

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه مازندران، بابلسر، ایران

2 گروه فیزیولوژی ورزش، دانشگاه آزاد ساری، ساری، ایران.

چکیده

سابقه و هدف: لیپوکالین-2 آدیپوکینی است که به میزان بالایی در بافت چربی سفید بیان می­شود و به نظر می­رسد بر متابولیسم گلوکز و حساسیت انسولینی اثرگذار باشد. هدف پژوهش حاضر بررسی تاثیر 8 هفته تمرین مقاومتی دایره­ای بر سطوح پلاسمایی لیپوکالین-2 مردان دیابتی بود.
مواد و روش­ها: شانزده مرد مبتلا به دیابت نوع 2 با میانگین سن 7.9 ± 49.2 سال، وزن 11.4 ± 81.6 کیلوگرم و شاخص توده بدن 3.9 ± 28.1 به طور تصادفی به دو گروه کنترل (تعداد = 8) و تمرین مقاومتی (تعداد = 8) تقسیم شدند. آزمودنی­های گروه تمرین 8 هفته تمرین مقاومتی دایره­ای با شدت 50 تا 80 درصد یک تکرار بیشینه را (3 روز در هفته) انجام دادند. شاخص­های پیکرسنجی و سطوح ناشتای گلوکز، انسولین و لیپوکالین-2 پلاسمایی همراه با هموگلوبین گلیکوزیله (HbA1c)، شاخص مقاومت انسولینی (HOMA-IR) و تعداد سلول­های سفید خون (WBC) در ابتدا و پایان مطالعه مورد ارزیابی قرار گرفت.
یافته­ها: تمرین مقاومتی موجب کاهش معنی­دار سطوح پلاسمایی انسولین، HbA1c و WBC مردان دیابتی شد (0.05>P). تغییرات غلظت پلاسمایی گلوکز و مقادیر HOMA-IR در گروه تمرین مقاومتی در مقایسه با گروه کنترل معنی­دار بود (0.05>P). غلظت پلاسمایی لیپوکالین-2 در گروه تمرین با افزایش همراه بود (0.05>P) که این تغییرات تفاوت معنی­داری در مقایسه با گروه کنترل داشت (0.05>P).
بحث و نتیجه­ گیری: یافته­های تحقیق حاضر بیانگر آن است که تمرین مقاومتی دایره­ای می­تواند روش مداخله­ی موثری در بهبود نیم­رخ متابولیکی و التهابی در مردان مبتلا به دیابت نوع 2 باشد. افزایش غلظت پلاسمایی لیپوکالین-2 احتمالاً ساز و کاری جبرانی در بهبود متابولیسم این بیماران است.

تازه های تحقیق

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کلیدواژه‌ها


عنوان مقاله [English]

Effect of Resistance Training on Plasma Lipocalin-2 Concentration, White Blood Cell Count, and Insulin Resistance Index in Type 2 Diabetic Men

نویسندگان [English]

  • Alireza Safarzade 1
  • Hasan Barzegari-Marzoni 2
  • Elahe Talebi-Garakani 1
  • Rozita Fathi 1
1 Department of exercise physiology, sport science faculty, University of Mazandaran, Babolsar, Iran
چکیده [English]

Background &Purpose: lipocalin-2 is a novel adipokine that is highly expressed by white adipose tissue and is likely to be effective on glucose metabolism and insulin sensitivity. The purpose of this study was to investigate the effect of eight weeks of circuit resistance training on plasma lipocalin-2 levels in diabetic men.
Methodology: Sixteen men with type 2 diabetes (age 49.2 ± 7.9 yr, weight 81.6 ± 11.4 kg, and BMI 28.1 ± 3.9) were randomly divided into control (n=8) and resistance training (n=8) groups. Subjects in training group performed eight weeks circuit resistance training with 50-80% 1RM (3 days/week). Anthropometrics parameters and fasting plasma glucose, insulin, and Lipocalin-2, as well as glycosylated haemoglobin (HbA1c), insulin resistance index (HOMA-IR), and white blood cell count (WBC) were measured at baseline and at the end of study.
Results: Resistance training induced significant reductions in fasting plasma insulin, HbA1c and WBC levels in diabetic men (P < 0.05). Significant changes in plasma glucose concentration and HOMA-IR scores were found when compared with control group (P < 0.05). Plasma lipocalin-2 concentration increased in training group (P < 0.05), and this alteration was statistically significant when compared with control group (P < 0.05).
Conclusion: Our findings showed that circuit resistance training can be an effective intervention to improve metabolic and inflammatory profile in type 2 diabetic men. Increased plasma concentrations of lipocalin-2 may represent a compensatory mechanism to improve the metabolism in these patients.

کلیدواژه‌ها [English]

  • Siderocalin
  • Resistance Training
  • Type 2 Diabetes
  • systemic Inflammation
##El-Mesallamy HO, Hamdy NM, Sallam AA. Effect of obesity and glycemic control on serum lipocalins and insulin-like growth factor axis in type 2 diabetic patients. Acta Diabetol. 2013; 50(5): 679-85.##Zimmet P, Alberti KG, Shaw J. Global and societal implications of the diabetes epidemic. Nature. 2001; 414(6865): 782-7.##Li L, Wang C, Bao Y, Wu H, Lu J, Xiang K, et al. Serum retinol-binding protein 4 is associated with insulin secretion in Chinese people with normal glucose tolerance. J Diabetes. 2009; 1(2): 125-30.##Fernández-Real JM, Ricart W. Insulin resistance and chronic cardiovascular inflammatory syndrome. Endocr Rev. 2003; 24(3): 278-301.##Choi KM, Lee JS, Kim EJ, Baik SH, Seo HS, Choi DS, et al. Implication of lipocalin-2 and visfatin levels in patients with coronary heart disease. Eur J Endocrinol. 2008; 158(2): 203-7.##Huang Y, Yang Z, Ye Z, Li Q, Wen J, Tao X, et al. Lipocalin-2, glucose metabolism and chronic low-grade systemic inflammation in Chinese people. Cardiovasc Diabetol. 2012; 11: 11.##Wang Y, Lam KS, Kraegen EW, Sweeney G, Zhang J, Tso AW, et al. Lipocalin-2 is an inflammatory marker closely associated with obesity, insulin resistance, and hyperglycemia in humans. Clin Chem. 2007; 53(1): 34-41.##Panidis D, Tziomalos K, Koiou E, Kandaraki EA, Tsourdi E, Delkos D, et al. The effects of obesity and polycystic ovary syndrome on serum lipocalin-2 levels: a cross-sectional study. Reprod Biol Endocrinol. 2010; 8: 151.##Mohebbi H, Khazaee MH, Esfahani M. The effect of aerobic exercise on blood glucose control, cardiorespiratory fitness and risk factors related to cardiovascular disease in mild and severe non-insulin dependent diabetic patients. Olympic Journal. 2006; 14(4): 17-24. (In Persian). ##Nakhzari K, Mogharnasi M, Haghighi A. Investigating the Response and Adaptation of Interleukin-15 to resistance training in non-athletic young men. Olympic Journal. 2011; 19(3): 71-80. (In Persian).##Irvine C, Taylor NF. Progressive resistance exercise improves glycaemic control in people with type 2 diabetes mellitus: a systematic review. Aust J Physiother. 2009; 55(4): 237-46.##Kang S, Woo JH, Shin KO, Kim D, Lee HJ, Kim YJ, et al. Circuit resistance exercise improves glycemic control and adipokines in females with type 2 diabetes mellitus. J Sports Sci Med. 2009; 8(4): 682-8.##Eriksson J, Taimela S, Eriksson K, Parviainen S, Peltonen J, Kujala U. Resistance training in the treatment of non-insulin-dependent diabetes mellitus. Int J Sports Med. 1997; 18(4): 242-6.##Honkola A, Forsén T, Eriksson J. Resistance training improves the metabolic profile in individuals with type 2 diabetes. Acta Diabetol. 1997; 34(4): 245-8.##Choi KM, Kim TN, Yoo HJ, Lee KW, Cho GJ, Hwang TG, et al. Effect of exercise training on A-FABP, lipocalin-2 and RBP4 levels in obese women. Clin Endocrinol (Oxf). 2009; 70(4): 569-74.##Damirchi A, Rahmani-Nia F, Mehrabani J. Lipocalin-2: Response to a Progressive Treadmill Protocol in Obese and Normal-weight Men. Asian J Sports Med. 2011; 2(1): 44-50.##Lee CT, Harris SB, Retnakaran R, Gerstein HC, Perkins BA, Zinman B, et al. White Blood Cell Subtypes, Insulin Resistance and β-cell Dysfunction in High Risk Individuals- the PROMISE Cohort. Clin Endocrinol (Oxf). 2014; 81(4): 536-41.##Hordern MD, Dunstan DW, Prins JB, Baker MK, Singh MA, Coombes JS. Exercise prescription for patients with type 2 diabetes and pre-diabetes: a position statement from Exercise and Sport Science Australia. J Sci Med Sport. 2012; 15(1): 25-31.##Soheili S, Gaeini A, Sori R. the effect of resistance training on systemic inflammation factors in old men. Olympic Journal. 2009; 19(3): 71-80. (In Persian).##Spiropoulos A, Goussetis E, Margeli A, Premetis E, Skenderi K, Graphakos S, et al. Effect of inflammation induced by prolonged exercise on circulating erythroid progenitors and markers of erythropoiesis. Clin Chem Lab Med. 2010; 48(2): 199-203.##Zhao P, Elks CM, Stephens JM. The induction of lipocalin-2 expression in vivo and in vitro. J Biol Chem. 2014; 289(9): 5960-9.##Yeh YH, Chang JL, Hsiao PC, Tsao SM, Lin CH, Kao SJ, et al. Circulating level of lipocalin 2 as a predictor of severity in patients with community-acquired pneumonia. J Clin Lab Anal. 2013; 27(4): 253-60.##Hopps E, Canino B, Caimi G. Effects of exercise on inflammation markers in type 2 diabetic subjects. Acta Diabetol. 2011; 48(3): 183-9.##Zhang J, Wu Y, Zhang Y, Leroith D, Bernlohr DA, Chen X. The role of lipocalin 2 in the regulation of inflammation in adipocytes and macrophages. Mol Endocrinol. 2008; 22(6): 1416-26.##Paton CM, Rogowski MP, Kozimor AL, Stevenson JL, Chang H, Cooper JA. Lipocalin-2 increases fat oxidation in vitro and is correlated with energy expenditure in normal weight but not obese women. Obesity (Silver Spring). 2013; 21(12): E640-8.##Goetz DH, Holmes MA, Borregaard N, Bluhm ME, Raymond KN, Strong RK. The neutrophil lipocalin NGAL is a bacteriostatic agent that interferes with siderophore-mediated iron acquisition. Mol Cell. 2002; 10(5): 1033-43.##Smith KD. Iron metabolism at the host pathogen interface: lipocalin 2 and the pathogen-associated iroA gene cluster. Int J Biochem Cell Biol. 2007; 39(10): 1776-80.##Albright A, Franz M, Hornsby G, Kriska A, Marrero D, Ullrich I, Verity LS. American College of Sports Medicine position stand. Exercise and type 2 diabetes. Med Sci Sports Exerc. 2000; 32(7): 1345-60.##Caro JF, Dohm LG, Pories WJ, Sinha MK. Cellular alterations in liver, skeletal muscle, and adipose tissue responsible for insulin resistance in obesity and type II diabetes. Diabetes Metab Rev. 1989; 5(8): 665-89.##DeFronzo RA, Simonson D, Ferrannini E. Hepatic and peripheral insulin resistance: a common feature of type 2 (non-insulin-dependent) and type 1 (insulin-dependent) diabetes mellitus. Diabetologia. 1982; 23(4): 313-9.##