پاسخ سطح سرمی عامل القایی هایپوکسی یک آلفا، فاکتور رشد اندوتلیال عروق و در صد اشباع مویرگی به یک جلسه تمرین شنای تناوبی زیر آبی (آپنه ای) در مردان جوان

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشیار فیزیولوژی ورزش، ، دانشگاه بیرجند، بیرجند، ایران (نویسنده مسئول)،

2 کارشناس ارشد فیزیولوژی ورزش، دانشگاه بیرجند، بیرجند، ایران

3 دانشجوی دکتری فیزیولوژی ورزش، دانشگاه بیرجند، بیرجند، ایران

چکیده

سابقه و هدف: سطوح سرمی عامل القایی هایپوکسی یک آلفا (HIF-1α) و عامل رشد اندوتلیال عروق (VEGF)  به عنوان فاکتورهای مهم در فرایند رگ زایی، با فعالیت ورزشی تغییر مى کنند. هدف از تحقیق حاضر، بررسی پاسخ سطوح سرمی HIF-1α، VEGF و درصد اشباع اکسیژن مویرگی محیطی (SpO2) متعاقب 1 جلسه تمرین شنای زیر آبی در مردان جوان بود.
مواد و روش ها: ده شناگر مرد (میانگین سن 26/2± 30/23 سال و شاخص توده بدنی 91/3± 80/23 کیلوگرم بر متر مربع)، داوطلبانه در این تحقیق شرکت کردند. تمرین شامل 20 متر شنای زیر آبی بود که 10 بار تکرار شد و بین هر 2 تکرار نیز 1 دقیقه استراحت در نظر گرفته شده بود. نمونه‌گیری خون، قبل، بلافاصله و 2 ساعت پس از اجرای تمرین انجام شد. میزان SpO2 قبل، بلافاصله پس از هر تکرار و 1 دقیقه بعد از اتمام تمرین اندازه‌‌گیری شد. داده‌ها با استفاده از آزمون آنالیز واریانس با اندازه‌گیری‌های مکرر و آزمون تعقیبی بونفرونی در سطح معناداری 05/0 P
یافته‌ها: یک جلسه تمرین شنای زیر آبی، موجب افزایش معنادارVEGF  سرم بلافاصله پس از تمرین شد (02/0P=) و 2 ساعت پس از تمرین به حدود سطح پایه بازگشت (67/0P=). سطح سرمیHIF-1α  بلافاصله پس از تمرین تغییر معناداری نداشت (99/0P=)، اما 2 ساعت پس از تمرین کاهش معناداری داشت (01/0P=). علاوه بر آن، تغییر معناداری در میزان SpO2 در هر 2 زمان پس از تمرین مشاهده نشد (05/0P=).  
نتیجه گیری: به نظر می رسد تمرینات شنای زیر آبی، با کاهش سطح سرمی HIF-1α  و افزایش سطح سرمی VEGF می تواند به عنوان یک عامل در شروع فرایندهای رگ زایی مشارکت داشته باشد و در جهت افزایش چگالی مویرگی در ورزشکاران مورد مطالعه قرار گیرد.

کلیدواژه‌ها


عنوان مقاله [English]

The response of serum levels of hypoxia inducible factor –1α, vascular endothelial growth factor and peripheral capillary oxygen saturation to one session of underwater swimming training (Apnea) in young men

نویسندگان [English]

  • . . 1
  • . . 2
  • . . 3
1
2
3
چکیده [English]

Background and aim: serum levels of hypoxia induced factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) as important factors in the process of angiogenesis, are change during exercise. The purpose of this study was assessment of the responsive serum levels of HIF-1α and VEGF and peripheral capillary oxygen saturation (SpO2), following one session underwater swimming in young men.
Methods: Ten male swimmers (mean age 23.3±2.26 years and BMI 23.8±3.91 kg/m²), voluntarily participated in this study. The exercise was repeated 10 times to 20 meters underwater swimming with a one minute rest between each repetition. Blood sampling, immediately and two hours after the exercise was done. SpO2 levels before, immediately after and one hour after the end of each workout was measured. The obtained data analyzed using analysis of variance with repeated measures and the Bonferroni post hoc test, with a significant level of P<0.05.
Results: A training, leading to a significant increase in serum level of VEGF immediately after exercise (P=0.02) and two hours after exercise returned to nearly baseline (P=0.67). Serum level of HIF-1α not significantly change after training (P=0.99), but the two hours after exercise had a significant reduction (P=0.01). In addition, no significant change was observed in the SpO2 in both time after exercise (P=0.05).
Conclusion: It seems that swimming underwater, with a reduction in serum levels of HIF-1α and elevation of serum VEGF, can contribute as a factor to be involved in the angiogenesis process and considered to increase capillary density in athletes.

کلیدواژه‌ها [English]

  • saturation
  • Swimming
  • Apnea
  • Hypoxia inducible factor –1α
  • Vascular Endothelial Growth Factor
  • Peripheral capillary oxygen
##Shimoda LA, Semenza GL. 2011. HIF and the lung: role of hypoxia-inducible factors in pulmonary development and disease. Am J Respir Crit Care Med. 183(2):152-6.##Weidemann A, Johnson RS. 2008. Biology of HIF-1alpha. Cell Death Differ. 15(4): 621-7.##Chun YS, Choi E, Kim GT, Choi H, Kim CH, Lee MJ. 2001. Cadmium blocks hypoxia-inducible factor (HIF)-1-mediated response to hypoxia by stimulating the proteasome dependent degradation of HIF 1alpha. Euro J Biochem. 267(13): 4198-204.##Tissot van Patot MC, Serkova NJ, Haschke M, Kominsky DJ. 2009. Enhanced leukocyte HIF-1alpha and HIF-1DNA binding in humans after rapid ascent to 4300 m. Free Radic Biol Med. 46(11):1551-7.##Lum JJ, Bui T, Gruber M, Gordan JD, DeBerardinis RJ, Covello KL, et al. 2007. The transcription factor HIF-1alpha plays a critical role in the growth factor-dependent regulation of both aerobic and anaerobic glycolysis. Genes Dev. 21(9):1037-49.##Mounier R, Pialoux V, Roels B, Thomas C, Millet G, Mercier J, et al. 2009. Effect of intermittent hypoxic training on HIF gene expression in human skeletal muscle and leukocytes. Eur J Appl Physiol. 105(4):515-24.##Thébaud B. 2007. Angiogenesis in lung development, injury and repair: implications for chronic lung disease of prematurity. Neonatology. 91(4):291-7.##Islami D, Bischof P, Chardonnens D. 2003. Modulation of placental vascular endothelial growth factor by leptin and hCG. Mol Hum Reprod. 9(7):395-8.##Davis PG, Wideman L, Bloomer RJ, Consitt LA, Weaver RA, You T. 2002. Acute effect of prolonged cycle ergometer exercise on plasma vascular endothelial growth factor. Med Sci Sports Exerc. 34(5): 30-5.##Wood RE, Sanderson BE, Askew CD, Walker PJ, Green S, Stewart IB. 2006. Effect of training on the response of plasma vascular endothelial growth factor to exercise in patients with peripheral arterial disease. Clin Sci (Lond).111(6):401-9.##DA Silva ND Jr, Fernandes T, Soci UP, Monteiro AW, Phillips MI, DE Oliveira EM. 2012. Swimming training in rats increases cardiac MicroRNA-126 expression and angiogenesis. Med Sci Sports Exerc. 44(8):1453-62.##Gu JW, Shparago M, Tan W, Bailey AP. 2006. Tissue endostatin correlates inversely with capillary network in rat heart and skeletal muscles. Angiogenesis. 9(2):93–9.##Suhr F, Brixius K, de Marees M, Bolck B, Kleinoder H, Achtzehn S, et al. 2007. Effects of short-term vibration and hypoxia during high-intensity cycling exercise on circulating levels of angiogenic regulators in humans. J Appl Physiol(1985). 103(2): 474-83.##Montesinos MC, Desai A, Chen JF, Yee H, Schwarzschild MA, Fink S, et al. 2003. Adenosine promotes wound healing and mediates angiogenesis in response to tissue injury via occupancy of A(2A) receptors. Am J Pathol. 160(6): 2009–18.##Gavin TP, Westerkamp M, Zwetsloot KA. 2006. Soleus, plantaris and gastrocnemius VEGF mRNA responses to hypoxia and exercise are preserved in aged compared with young female C57BL/6 mice. Acta Physiol(Oxf). 188(2): 113–21.##Jensen L, Schjerling P, Hellsten Y. 2004. Regulation of VEGF and bFGF mRNA expression and other proliferative compounds in skeletal muscle cells. Angiogenesis. 7(3): 255–67.##Jewell UR, Kvietikova I, Scheid A, Bauer C, Wenger RH, Gassmann M. 2001. Induction of HIF-1 in response to hypoxia is instantaneous. FASEB J. 15(7): 1312–4.##Richardson RS, Duteil S, Wary C, Wray DW, Hoff J, Carlier PG. 2006. Human skeletal muscle intracellular oxygenation: the impact of ambient oxygen availability. J Physiol. 571(Pt 2): 415–24.##Mason SD, Howlett RA, Kim MJ, Olfert IM, Hogan MC, McNulty W, et al. 2004. Loss of skeletal muscle HIF-1alpha results in altered exercise endurance. PLoS Biol. 2(10): e288.##Schagatay E, Van Kampen M, Emanuelsson S, Holm B. 2000. Effects of physical and apnea training on apneic time and the diving response in humans. Eur J Appl Physiol. 82(3): 161–9.##Joulia F, Steinberg JG, Faucher M, Jamin T, Ulmer C, Kipson N, et al. 2003. Breath-hold training of humans reduces oxidative stress and blood acidosis after static and dynamic apnea. Respir Physiol Neurobiol. 137(1): 19–27.##Stefanini MO, Wu FT, Mac Gabhann F, Popel AS. 2008. A compartment model of VEGF distribution in blood, healthy and diseased tissues. BMC Syst Biol. 2: 77.##Ramsbottom R, Brewer J, Williams C. 1988. A progressive shuttle run test to estimate maximal oxygen uptake. Br J Sports Med. 22(4): 141-8.##Frigato E, Lunghi L, Ferretti ME, Biondi C, Bertolucci C. 2009. Evidence for circadian rhythms in human trophoblast cell line that persist in hypoxia. Biochem Biophys Res Commun. 378(1): 108-11.##Carmeliet P. 2003. Angiogenesis in health and disease. Nat Med. 9(6): 653-60.##Dill DB, Costill DL. 1974. Calculation of percentage changes in volumes of blood, plasma, and red cells in dehydration. J Appl Physiol. 37(2): 247-8.##Eidi Yusef Abad H, Saghebjoo M, Hedayati M, Ilbeigi S. 2015. The response of serum levels of vascular endothelial growth factor to two types of swimming exercise in hypoxia condition in young men. Sci J Kurdistan Univ Med Sci. 20 (3): 10-22. (Persian)##Buford TW, Cooke MB, Shelmadine BD, Hudson GM, Redd L, Willoughby DS. 2009. Effects of eccentric treadmill exercise on inflammatory gene expression in human skeletal muscle. Appl Physiol Nutr Metab. 34(4):745-53.##Lee JH, Chun T, Park SY, Rho SB. 2008. Interferon regulatory factor-1 (IRF-1) regulates VEGF-induced angiogenesis in HUVECs. Biochim Biophys Acta .1783(9): 1654 -62.##Haddad JJ, Harb HL. 2005. Cytokines and the regulation of hypoxia-inducible factor (HIF)-1alpha. Int Immunopharmacol. 5(3):461-83.##Rundqvist H. 2008. Skeletal muscle HIF-1 and exercise. Thesis for doctoral degree. Stockholm: Karolinska Institutet. ## Pugh CW, Ratcliffe PJ. 2003. Regulation of angiogenesis by hypoxia: role of the HIF system. Nat Med. 9(6):677-84.##Gustafsson T, Ameln H, Fischer H, Sundberg CJ, Timmons JA, Jansson E. 2005. VEGF-A splice variants and related receptor expression in human skeletal muscle following submaximal exercise. J Appl Physiol(1985). 98(6):2137–46.##Yeh CH, Cho W, So EC, Chu CC, Lin MC, Wang JJ, et al. 2011. Propofol inhibits lipopolysaccharide-induced lung epithelial cell injury by reducing hypoxia-inducible factor-1α expression. Br J Anaesth. 106 (4):590-9.##Lusina SJ, Kennedy PM, Inglis JT, McKenzie DC, Ayas NT, Sheel AW. 2006. Long-term intermittent hypoxia increases sympathetic activity and chemosensitivity during acute hypoxia in humans. J Physiol. 575(Pt 3):961–70.##Truijens MJ, Toussaint HM, Dow J, Levine BD. 2003. Effect of high-intensity hypoxic training on sea-level swimming performances. J Appl Physiol(1985). 94(2):733–43.##Woorons X, Bourdillon N, Lamberto C, Vandewalle H, Richalet JP, Mollard P, et al. 2011. Cardiovascular responses during hypoventilation at exercise. Int J Sports Med. 32(6): 438–45.##