The effect of high intensity interval training in normoxia and hypobaric hypoxia situation on the expression of genes involved in liver mitophagy in male Wistar rats fed with high fat diet

Document Type : Research Paper

Authors

Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Guilan, Rasht, Iran

10.22080/jaep.2024.26404.2169

Abstract

Objectives: Consuming a high-fat diet leads to dysfunction of liver mitochondria and the occurrence of fatty liver disorders, and mitophagy, which plays a role in removing damaged mitochondria, can probably be effective in improving non-alcoholic fatty liver disease. The aim of this study was to determine the effect of nutrition, training and hypoxia on liver mitophagy in male Wistar rats
Methods: Thirty-two male rats were randomly divided into four groups of eight including normal diet (ND), high-fat diet (HFD), high-fat diet and training in normoxia (HFD-HIIT) and high-fat diet and training in hypoxia (HFD-HHIIT). After determining the maximum aerobic velocity (MAV) in normoxia and hypoxia-hypobaric, the HIIT protocol was performed for 12 weeks and three sessions per week, which included 3 to 8 bouts 4-minute activity with an intensity of 80 to 93 percent of MAV and 2-minute active rest periods with an intensity of 50 percent of MAV. At the end, the levels of HIF-1α, Parkin and Fundc1 genes were measured through RT-PCR
Results: The expression of Parkin and Fundc1 genes in HFD-HIIT and HFD-HHIIT groups increased significantly compared to HFD (P<0.05). Also, the HFD-HHIIT group showed a significant increase compared to the HFD-HIIT group (P<0.05). Also, liver fat content and HIF-1α gene expression showed a significant decrease in HFD-HIIT and HFD-HHIIT groups compared to HFD (P<0.05).
Conclusion: It seems that training in hypoxic conditions compared to normoxia has an additional effect on increasing liver mitophagy and subsequently controlling liver fat content.

Keywords


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