The effect of four weeks of incremental endurance training on sarcolipin, FNDC5 and PGC1α proteins in Soleus and extensor digitorum longus of male Wistar rats

Document Type : Research Paper

Authors

1 Department of Sport Physiology, Faculty of Sport Sciences, Shahid Beheshti University, Tehran, Iran

2 Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

10.22080/jaep.2022.22367.2069

Abstract

Background & Purpose: The aim of this study was to determine the effect of four weeks of incremental endurance training on Sarcolipin (SLN), FNDC5, and PGC1α protein levels in the soleus (SOL) and extensor digitorum longus (EDL) muscle of adult male Wistar rats.
Methodology: In this experimental study, 14 adult male Wistar rats were divided into two groups of control (n=7) and training (n=7). The training group performed endurance exercises for 4 weeks and 5 sessions per week, which included running at the intensity of 75 to 80% of the Vo2max. In rat surgery, SOL and EDL muscles were extracted, followed by changes in SLN proteins, and intramuscular FNDC5 and PGC1α were measured by Western blotting technic. Data were statistically analyzed by independent t-test (P≤0.05).
Results: The results of statistical analysis showed that four weeks of incremental endurance training caused significant changes in the amounts of SLN (P= 0.035), FNDC5 (P= 0.02), and PGC1α (P = 0.012) proteins. In the soleus muscle, compared to the control group. Also, in EDL muscle, significant changes were observed in the amounts of SLN (P= 0.01) and FNDC5 (P = 0.043) proteins in comparison with the control group, but in the changes of PGC1α protein in EDL muscle compared to the control group, no significant difference was observed (P= 0.17).
Conclusion: According to the parameters of the study, it seems that endurance exercise can increase the amount of proteins related to thermogenesis and metabolism.

Keywords


##Bal NC, Gupta SC, Pant M, Sopariwala DH, Gonzalez-Escobedo G, Turner J, et al. Is Upregulation of Sarcolipin Beneficial or Detrimental to Muscle Function? Frontiers in physiology. 2021;12:99. ##Bal NC, Periasamy M. Uncoupling of sarcoendoplasmic reticulum calcium ATPase pump activity by sarcolipin as the basis for muscle non-shivering thermogenesis. Philosophical Transactions of the Royal Society B. 2020;375(1793):20190135. ##Bedford TG, Tipton CM, Wilson NC, Oppliger RA, Gisolfi CV. Maximum oxygen consumption of rats and its changes with various experimental procedures. Journal of Applied Physiology. 1979;47(6):1278-83. ##Bombardier E. The role of sarcolipin in calcium handling and obesity. 2010##Gamu D, Trinh A, Fajardo VA, Bombardier E, Tupling AR. Sarcolipin expression is not required for the mitochondrial enzymatic response to physical activity or diet. Journal of Applied Physiology. 2017;122(5):1276-83. ##Haram PM, Kemi OJ, Lee SJ, Bendheim MØ, Al-Share QY, Waldum HL, et al. Aerobic interval training vs. continuous moderate exercise in the metabolic syndrome of rats artificially selected for low aerobic capacity. Cardiovascular research. 2009;81(4):723-32. ##Hoppeler H, Howald H, Conley K, Lindstedt SL, Claassen H, Vock P, et al. Endurance training in humans: aerobic capacity and structure of skeletal muscle. Journal of applied physiology. 1985;59(2):320-7. ##Kahan LG, Mehrzad R. Environmental factors related to the obesity epidemic.  Obesity: Elsevier; 2020. p. 117-39. ##Lizamore CA, Hamlin MJ. The use of simulated altitude techniques for beneficial cardiovascular health outcomes in nonathletic, sedentary, and clinical populations: a literature review. High altitude medicine & biology. 2017;18(4):305-21. ##Loellgen H, Zupet P, Bachl N, Debruyne A. Physical activity, exercise prescription for health and home-based rehabilitation. Sustainability. 2020;12(24):10230. ##Maliszewska K, Kretowski A. Brown Adipose Tissue and Its Role in Insulin and Glucose Homeostasis. International Journal of Molecular Sciences. 2021;22(4):1530. ##Maurya SK, Bal NC, Sopariwala DH, Pant M, Rowland LA, Shaikh SA, et al. Sarcolipin is a key determinant of the basal metabolic rate, and its overexpression enhances energy expenditure and resistance against diet-induced obesity. Journal of Biological Chemistry. 2015;290(17):10840-9. ##Morales‐Alamo D, Martinez‐Canton M, Gelabert‐Rebato M, Martin‐Rincon M, de Pablos‐Velasco P, Holmberg HC, et al. Sarcolipin expression in human skeletal muscle: Influence of energy balance and exercise. Scandinavian journal of medicine & science in sports. 2020;30(3):408-20. ##Norris SM, Bombardier E, Smith IC, Vigna C, Tupling AR. ATP consumption by sarcoplasmic reticulum Ca2+ pumps accounts for 50% of resting metabolic rate in mouse fast and slow twitch skeletal muscle. American Journal of Physiology-Cell Physiology. 2010;298(3):C521-C9. ##Park JH, Moon JH, Kim HJ, Kong MH, Oh YH. Sedentary lifestyle: Overview of updated evidence of potential health risks. Korean journal of family medicine. 2020;41(6):365. ##Reguero M, Gómez de Cedrón M, Wagner S, Reglero G, Quintela JC, Ramírez de Molina A. Precision Nutrition to Activate Thermogenesis as a Complementary Approach to Target Obesity and Associated-Metabolic-Disorders. Cancers. 2021;13(4):866. ##Rodrigues B, Figueroa DM, Mostarda CT, Heeren MV, Irigoyen M-C, De Angelis K. Maximal exercise test is a useful method for physical capacity and oxygen consumption determination in streptozotocin-diabetic rats. Cardiovascular diabetology. 2007;6(1):1-7. ##Sopariwala D, Shaikh S, Pant M, Rowland L, Periasamy M. Sarcolipin overexpression in mice increases their endurance exercise capacity (1162.2). The FASEB Journal. 2014;28:1162.2. ##Trinh A. Effects of Sarcolipin Ablation on Mitochondrial Enzyme Adaptations to Exercise Training: University of Waterloo; 2013. ##Vogt ÉL, Von Dentz MC, Rocha DS, Argenta Model JF, Kowalewski LS, de Souza SK, et al. Metabolic and Molecular Subacute Effects of a Single Moderate-Intensity Exercise Bout, Performed in the Fasted State, in Obese Male Rats. International journal of environmental research and public health. 2021;18(14):7543. ##Maurya SK, Herrera JL, Sahoo SK, Reis FC, Vega RB, Kelly DP, et al. Sarcolipin signaling promotes mitochondrial biogenesis and oxidative metabolism in skeletal muscle. Cell reports. 2018;24(11):2919-31. ##Sopariwala DH, Pant M, Shaikh SA, Goonasekera SA, Molkentin JD, Weisleder N, et al. Sarcolipin overexpression improves muscle energetics and reduces fatigue. Journal of Applied Physiology. 2015;118(8):1050-8. ##