The effect of MCT1 T1470A polymorphism on the number of repetition and the volume of resistance exercise in young male athletes

Document Type : Research Paper

Authors

1 Department of Physical Education, Faculty of Humanities and Social Science,, University of Kurdistan, Sanandaj, Iran,

2 Department of physical education and sport sciences, Islamic Azad University, Marivan Branch, Marivan, Iran,

3 Department of Physical Education, Faculty of Humanities and Social Science,, University of Kurdistan, Sanandaj, Iran

10.22080/jaep.2021.21794.2046

Abstract

Background & Purpose: The aim of this study was to investigate the effect of MCT1 T1470A polymorphism on the number of repetitions and the volume of resistance exercise in young athletes in endurance-semi-endurance and speed-strength sports.
Methodology: 49 trained young men [endurance semi-endurance group (21 n); strength-speed group (28 n)] entered the study with the inclusion criteria (178.57 91 8.91 cm, 75.34 37 13.37 kg, Year 45/5 ± 2/24). In five days, anthropometric indices were measured, sports tests (chest and squat press with 1RM 65%, 3 cycles, repetition to exhaustion and 90 seconds rest between periods) and blood sampling were performed. After DNA extraction from blood samples, ARMS-PCR method was used to determine the genotype of the samples. Chi-square tests were used to evaluate Hardy-Weinberg equilibrium and one-way ANOVA and Kruskal-Wallis tests with a significance level of p≤0.05 to investigate the effect of different genotypes of MCT1T14701 polymorphism on the number of replications and volume of resistance exercise
Results: In examining the difference between the means of three genotypes AA, TT and AT, a significant difference was found between AA genotype and the other two genotypes (p=0.05) in the endurance-semi-endurance group in terms of volume of resistance chest press and the highest mean (323.85 ± 2209.00 kg in three periods) belonged to AA genotype. In terms number of repetitions, although the mean of subjects with AA genotype in both groups and for both chest and squat tests was higher than the other two genotypes, none of these differences were statistically significant
Conclusion: The results of the study illustrated that endurance-semi-endurance athletes with A allele and AA genotype show a better function in the volume of resistance exercise in the pectoral girdle muscles as well as the upper limbs muscles in comparison to the athletes with T allele and TT and AT genotypes.

Keywords


##Al-haggar, M., Eid, A., & Ramadan, W. MCT1 polymorphism among Egyptian children and adolescents as a useful predictor for physical fitness and muscle fatigue. J Syst Biol Proteome Res. 2017; 1 (2): 1-7 J Syst Biol Proteome Res 2017 Volume 1 Issue, 1, 60-65##Al-Lami, H. A. A., Khaleel, S. H., & Yonis, S. D. (2020). Study the correlation between alleles of MCT1 gene and enduring performance in handball players. ##Ben-Zaken, S., Meckel, Y., Nemet, D., Kassem, E., & Eliakim, A. (2021). Genetic Basis for the Dominance of Israeli Long-Distance Runners of Ethiopian Origin. The Journal of Strength & Conditioning Research, 35(7), 1885-1896. ##Brooks, G. A. (2009). Cell–cell and intracellular lactate shuttles. The Journal of physiology, 587(23), 5591-5600. ##Brzycki, M. (1993). Strength Testing—Predicting a One-Rep Max from Reps-to-Fatigue. Journal of Physical Education, Recreation & Dance, 64(1), 88-90. ##Buitrago, S., Wirtz, N., Yue, Z., Kleinöder, H., & Mester, J. (2013). Mechanical load and physiological responses of four different resistance training methods in bench press exercise. The Journal of Strength & Conditioning Research, 27(4), 1091-1100.##Cupeiro, R., González-Lamuño, D., Amigo, T., Peinado, A. B., Ruiz, J. R., Ortega, F. B., & Benito, P. J. (2012). Influence of the MCT1-T1470A polymorphism (rs1049434) on blood lactate accumulation during different circuit weight trainings in men and women. Journal of science and medicine in sport, 15(6), 541-547.##Cupeiro, R., Pérez-Prieto, R., Amigo, T., Gortázar, P., Redondo, C., & González-Lamuño, D. (2016). Role of the monocarboxylate transporter MCT1 in the uptake of lactate during active recovery. European journal of applied physiology, 116(5), 1005-1010.##Fedotovskaya, O. N., Mustafina, L. J., Popov, D. V., Vinogradova, O. L., & Ahmetov, I. I. (2014). A common polymorphism of the MCT1 gene and athletic performance. International journal of sports physiology and performance, 9(1), 173-180. ##Gladden, L. (2004). Lactate metabolism: a new paradigm for the third millennium. The Journal of physiology, 558(1), 5-30. ##John, R., Dhillon, M. S., & Dhillon, S. 2020). Genetics and the elite athlete: our understanding in 2020. Indian journal of orthopaedics, 54(3), 256-263. ##Joyner, M. J. (2019). Genetic approaches for sports performance: how far away are we? Sports Medicine, 1-6. ##Kikuchi, N., Fuku, N., Matsumoto, R., Matsumoto, S., Murakami, H., Miyachi, M., & Nakazato, K. (2017).The association between MCT1 T1470A polymorphism and power-oriented athletic performance. International journal of sports medicine, 38(01), 76-80. ##Lacerda, L. T., Martins-Costa, H. C., Diniz, R. C., Lima, F. V., Andrade, A. G., Tourino, F. D., Bemben, M. G., & Chagas, M. H. (2016). Variations in repetition duration and repetition numbers influence muscular activation and blood lactate response in protocols equalized by time under tension. The Journal of Strength & Conditioning Research, 30(1), 251-258. ##Lima, F. V., Costa, C. G., Lacerda, L. T. d., Silva, L. V. R. d., Diniz, R. C. R., Costa, H. C. M., Tourino, F. D., & Chagas, M. H. (2017). Effect of resistance exercise with different muscle action durations on lactate response. Journal of Physical Education, 28. ##Lippi, G., Longo, U. G., & Maffulli, N. (2010). Genetics and sports. British medical bulletin, 93(1), 27-47. ##Maciejewska-Skrendo, A., Cięszczyk, P., Chycki, J., Sawczuk, M., & Smółka, W. (2019). Genetic Markers Associated with Power Athlete Status. Journal of human kinetics, 68, 17. ##Massidda, M., Eynon, N., Bachis, V., Corrias, L., Culigioni, C., Cugia, P., Scorcu, M., & Calò, C. M. (2016). Association between MCT1 A1470T polymorphism and fat-free mass in well-trained young soccer players. The Journal of Strength & Conditioning Research, 30(4), 1171-1176. ##Onali, F., Calò, C. M., Massidda, M., Álvarez-Álvarez, M. M., & Esteban, M. E. (2018). An unexpected world population variation of MCT1 polymorphism 1470T> A involved in lactate transport. European journal of sport science, 18(10), 1376-1382. ##Pinto, R. R., Karabulut, M., Poton, R., & Polito, M. D. (2018). Acute resistance exercise with blood flow restriction in elderly hypertensive women: haemodynamic, rating of perceived exertion and blood lactate. Clinical physiology and functional imaging, 38(1), 17-24. #Saito, M., Ginszt, M., Massidda, M., Cięszczyk, P., Okamoto, T., Majcher, P., Nakazato, K., & Kikuchi, N. (2021). Association between MCT1 T1470A polymorphism and climbing status in Polish and Japanese climbers. Biology of Sport, 38(2), 229. ##Sawczuk, M., Banting, L. K., Cięszczyk, P., Maciejewska-Karłowska, A., Zarębska, A., Leońska-Duniec, A., Jastrzębski, Z., Bishop, D. J., & Eynon, N. (2015). MCT1 A1470T: a novel polymorphism for sprint performance? Journal of science and medicine in sport, 18(1), 114-118. ##Souza, J., Paz, G., & Miranda, H. (2017). Blood lactate concentration and strength performance between agonist-antagonist paired set, superset and traditional set training. Archivos de medicina del deporte: revista de la Federación Española de Medicina del Deporte y de la Confederación Iberoamericana de Medicina del Deporte(179), 145-150. ##Thomas, C., Perrey, S., Lambert, K., Hugon, G., Mornet, D., & Mercier, J. (2005). Monocarboxylate transporters, blood lactate removal after supramaximal exercise, and fatigue indexes in humans. Journal of applied physiology, 98(3), 804-809. ##Willardson, J. M. (2008). A Brief Review: How Much Rest between Sets? Strength & Conditioning Journal.30(3), 44-50 ##Wirtz, N., Wahl, P., Kleinöder, H., & Mester, J. (2014). Lactate kinetics during multiple set resistance exercise. Journal of sports science & medicine, 13(1), 73.  ##