The effect of 6 weeks progressive aerobic training with consumption of vitamin D3 on nerve growth factor levels in streptozotocin-Induced diabetic rat’s hippocampus

Document Type : Research Paper

Authors

1 Department of Sports Physiology, Islamic Azad University, Sari Branch

2 Department of Sport Physiology, Islamic Azad University, Sari Branch

3 Professor of Cellular and Molecular Research Center, Babol University of Medical Sciences

Abstract

Background&purpose:The purpose of this study was investigate the effect of 6 weeks treadmill running with consumption of vitamin D3 on nerve growth factor levels in streptozotocin-Induced diabetic rat’s hippocampus
Methodology:48rats(weight: 220-240 g and age: 9week)were divided into 6 groups:control,sham،control diabetes, exercise + diabetes, vitamin D3 + diabetes, vitamin D3 + exercise+diabetes.The exercise protocol was treadmill running for 5 days per week for 6 weeks. The groups with vitamin D3supplementation treatment received a dose of 1 μg / kg vitamin D3 intraperitoneal vitamin D3 for 6 weeks, two days per week for first two weeks and then once a week in the last four weeksDiabetes was induced by injection of streptozotocin(60 mg / kg)intraperitoneally. NGF level in the hippocampus were measured by ELISA
Results: In the present study, the level of NGFin the combined group was significantly increased compared with the control group (p=0.0001)Also, there was a significant increase in supplementation and diabetes in comparison with the combined group (p=0.0001) On the other hand, there was no significant difference in the exercise group and diabetes compared to the combination group (P>0.05)
Conclusion: Overall, the results showed that NGF levels in the exercise group, vitamin and also in the combination group, as compared to the control group, increased significantly, which was more pronounced in the vitamin group.Therefore, it seems that each of the interventions, especially the intake of vitamin D3, can be a major factor in the protection of the central nervous system against diabetes due to an increase in neurotrophic factors

Keywords

References

##Havilah P, Pandit Vinodh B, Durga Prasad K. 2013. Adenosine Deaminase Activity in Type-2 Diabetes Mellitus – An Independent Marker of Glycemic Status and Stimulator of Lipid Peroxidation. Int. J. Chem, 02 (06), 1175-1178.##Knopman D, Boland LL, Mosley T, Howard G, Liao D, Szklo M, et al. 2001. Atherosclerosis Risk in Communities (ARIC) Study Investigators.Cardiovascular risk factors and cognitive decline in middle-aged adults. Neurology. 56: PP: 42–8.##Gispen WH, Biessels GJ. 2000. Cognition and synaptic plasticity in diabetes mellitus.Trends Neurosci.  23: PP: 542–9.##Sun MK, Alkon DL. 2006. Link between Alzheimer’s disease and diabetes. Drugs Today. 42: PP: 481-489.##Baydas G, Nedzvetskii VS, Nerush PA, Kirichenko SV, Yoldas T. 2003. Altered expression of NCAM in hippocampus and cortex may underlie memory and learning deficits in rats with streptozocin-induced diabetes mellitus. Life Science. 73: 1907-16.##Pedersen BK, Pedersen M, Krabbe KS, Bruunsgaard H, Matthews VB, Febbraio MA. 2009. Role of exercise-induced bdnf production in the regulation of energy homeostasis. Exp Physiol . 94: 1153-1160.##Fang Yu, Kolanowski Ann. 2009. Facilitating Aerobic Exercise Training in older adults with Alzheimer s disease. Geriatr Nurs. Vol 30, 4, 250-259.##Winter B, Breitenstein C, Mooren FC, Voelker K, Fobker M, Lechtermann A. 2007. High impact ruuning improves learning. Neurobiol Learn Mem . 87: 597-609.##Momeni, z. Behnam rasoli, m. Feridoni, m. 2011. Comparison of the effects of type 1 and type 2 diabetes on hippocampal neuronal density in male Wistar rats. Ir J Diabetes and Lipid. Farvardin. Volume 10 (Number 4): 339-346. (In Persian).##Reagan LP. 2007. Insulin signaling effects on memory and mood. Curr Opin Pharmacol . 7: 633-7.##Jafari anarkooli i, Sankian m, Varasteh a.r, Haghir h. 2010. Effects of insulin and ascorbic acid on inhibition of the apoptosis in hippocampus of stz-induced diabetic rats. Anat Sci J. 7: 133-143.##Xu B, Zang K, Ruff NL, Zhang YA, McConnell SK, Stryker MP. 2000. Cortical degeneration in the absence of neurotrophin signaling: dendritic retraction and neuronal loss after removal of the receptor TrkB. Neuron. 26: 233-45.##Hamidi Perchikolaei. S. O, Falah Mohamadi. Z, Hajizadeh Moghadam. A. 2016. The effect of treadmill running with consumption of vitamin D3 on NGF levels in Parkinsonian rat’s striatum. Sport Physiology. Spring. 8 (29): 91-102. (In Persian).##Delcroix JD, Michael GJ, Priestley JV, Tomlinson DR, Fernyhough P. 1998. Effect of nerve growth factor treatment on p75NTR gene expression in lumbar dorsal root ganglia of streptozocin-induced diabetic rats. Diabetes.47:1779-85.##Chae C-H, Jung S-L, An S-H, Jung C-K, Nam S-N, Kim H-T. 2011. Treadmill exercise suppresses muscle cell apoptosis by increasing nerve growth factor levels and stimulating pphosphatidylinositol 3-kinase activation in thesoleus of diabetic rats. J Physiol Biochem .67:235-41.##Aloe L, Rocco M, Bianchi P, Manni L. 2012. Nerve growth factor: from the early discoveries to the potential clinical use. Journal of Translational Medicine; 10:239.##De Salles Bf, Simão R, Fleck SJ, Dias I, Kraemer-Aguiar LG, Bouskela E.2010. Effects of resistance training on cytokines. Int J Sports Med. 31(7):441-50.##Tang Z, Yuan L, Gu C, Liu Y, Zhu L. 2005. Effect of exercise on the expression of adiponectin mRNA and GLUT4 mRNA in type 2 diabetic rats. J Huazhong Univ Sci Technolog Med Sci. 25(2):191-193.##Priscylla N, Pamela B, Fabiana G, Larissa B, Patrícia, S. 2017. Physical exercise reverses spatial memory deficit and induces hippocampal astrocyte plasticity in diabetic rats. Brain Res. Volume 1655, 15 January, Pages 242-251.##Cotman C. W, Berchtold N. C, Christie L. A. 2007. Exercise builds brain health: key roles of growth factor cascades and inflammation. Trends Neurosci. 30(9), 464-472.##Eyles D. W, Burne T. H, McGrath J. 2012. Vitamin D, effects on brain development, adult brain function and the links between low levels of vitamin D and neuropsychiatric disease. Front Neuroendocrinol. Vol 34, 1. 47-64.##Kalueff A, Minasyan A, Tuohimaa P. 2005. Anticonvulsant effects of 1, 25-dihydroxyvitamin D in chemically induced seizures in mice. Brain Res Bull. 67(1), 156-160.##Cornet C.  Baudet   L, Neveu A. 1998. 1, 25-Dihydroxyvitamin D3 regulates the expression of VDR and NGF gene in Schwann cell in vitro. J Neurosci Res. vol. 53, no. 6, pp. 742-746.##Riaz S, Malcangio M, Miller M, Tomlinson D R.1999. A vitamin D3 derivative (CB1093) induces nerve growth factor and prevents neurotrophic deficits in streptozotocin-diabetic rats. Diabetologia. 42(11): 1308-13.##Pittas A, Dawson B, Li T, Willett W, Manson J, Hu F. 2006. Vitamin D and Calcium Intake in Relation to Type 2 Diabetes in Women. Diabetes Care, Vol 29, 3, 650-656.##Chae, C.H., Jung, S.L., An, S.H., Park, B.Y., Wang, S.W., Cho, I.H., Cho, J.Y., Kim, H.T.2009. Treadmill exercise improves cognitive function and facilitates nerve growth factor signaling by activating mitogen-activated protein kinase/ extracellular signalregulated kinase1/2 in the streptozotocin-induced diabetic rat hippocampus. Neuroscience; 164, 1665–1673.##Moradi A, Mohammadi S, Hamidi Alamdari D.2015. Effect of adipose tissue-derived stem cells on the control of the blood glucose level in diabetic rats. J Shahid Sadoughi Univ Med Sci . 23(8): 717-26. (In Persian).##Chang-Hun, Chae. Hyun-Tae, Kin. 2009. Forced moderate-intensity treadmill exercise suppresses appotosis by increasing the level of NGF and stimulating phosphatidy linositol 3-kinase signaling in the hippocampus of induced aging rats. Neurochem Int. Vol 55, 4, 208-213.##Chae C-H, Lee H, Jung S, Kim T, Kim J, and Kim N. RETRACTED. 2012.  Swimming exercise increases the level of nerve growth factor and stimulates neurogenesis in adult rat hippocampus. Neuroscience. 212: p. 30-37.##Fernandes D, Eyles D. 2009. Feron, Vitamin D, a neuro-immunomodulator: implications for neurodegenerative and autoimmune diseases. Psychoneuroendocrinology. 34: p. S265-S277.##Yeylaghi Ashrafi M, Dabidi Roshan V. 2016.  Aerobic and Anaerobic Exercise of the Acute and Chronic and the Selected Markers of Oxidative Stress: A Systematic Review in Human and Animal Studies. Journal of Sabzevar University of Medical Sciences. Volume 22, Number 7. 1126-1138. (in Persian).##Calcutt NA, Jorge MC, Yaksh TL, Chaplan SR.1996. Tactile allodynia and formalin hyperalgesia in streptozotocin-diabetic rats: effects of insulin, aldose reductase inhibition and lidocaine. Pain; 68: 293-299.##
Journal of Applied Exercise Physiology
Volume 14, Issue 28
February 2019
Pages 191-200

Files

History

  • Receive Date: 14 February 2018
  • Revise Date: 04 April 2018
  • Accept Date: 06 May 2018

Share

How to cite

Statistics