Background and objective: Forced running wheel is a commonly used ergometer in animal studies. Given the importance of determining exercise intensity in training protocols, the aim of this study was to determine the intensity of exercise corresponding to the maximal lactate steady state (MLSS) in Wistar rats using forced running wheel to prescribe the appropriate exercise intensity. Method and materials: After a three-week period of familiarization with forced running wheel, 21 male Wistar rats (200 ± 20 g) were randomly assigned to four groups including incremental-power test (n = 6), constant-power test at 14.5 m/min (n = 5), constant-power test at 16 m/min, and constant-power test at 17.5 m/min. The highest intensity of effort inducing an increase in blood lactate concentration of < 1 mmol/L over 10th to 25th minutes of constant-intensity test was considered MLSS. Results: Blood lactate concentrations at velocities of 14.5 m/min and 16 m/min reached a steady state during 10th to 25th minutes of exercise, while the velocity of 17.5 m/min was characterized by a constant increase in blood lactate concentration. Therefore, the intensity of exercise at velocity of 16 m/min, with mean lactate concentration of 3.85 ± 0.91 mol/L, was considered MLSS. Conclusion: Our results showed that Wistar rats reach MLSS at 16 m/min on forced running wheel. The findings of this study could contribute significantly to design of training protocols using forced running wheel in Wistar rats for future research.
Rezaei, S., Agha-Alinejad, H., & Molanouri Shamsi, M. (2017). Determination of the Maximal Lactate Steady State Intensity in Wistar Rats on Forced Running Wheel. Journal of Applied Exercise Physiology, 13(25), 175-186. doi: 10.22080/jaep.2017.1597
MLA
Sajjad Rezaei; Hamid Agha-Alinejad; Mahdiyeh Molanouri Shamsi. "Determination of the Maximal Lactate Steady State Intensity in Wistar Rats on Forced Running Wheel". Journal of Applied Exercise Physiology, 13, 25, 2017, 175-186. doi: 10.22080/jaep.2017.1597
HARVARD
Rezaei, S., Agha-Alinejad, H., Molanouri Shamsi, M. (2017). 'Determination of the Maximal Lactate Steady State Intensity in Wistar Rats on Forced Running Wheel', Journal of Applied Exercise Physiology, 13(25), pp. 175-186. doi: 10.22080/jaep.2017.1597
VANCOUVER
Rezaei, S., Agha-Alinejad, H., Molanouri Shamsi, M. Determination of the Maximal Lactate Steady State Intensity in Wistar Rats on Forced Running Wheel. Journal of Applied Exercise Physiology, 2017; 13(25): 175-186. doi: 10.22080/jaep.2017.1597
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