The effects of vitamin e and aerobic trainings on blood lactate, ldh enzyme and exhaustion time in untrained men

Document Type : Research Paper

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Abstract

The effects of vitamin E and aerobic training on the relationship between blood lactate, lactate dehydrogenize (LDH) enzyme and exhaustion is doubtful.Therefore, this study was designed to investigate the interaction effects of vitamin E and aerobic training (55-75% HRR)to find out about the association between theses variables. For this purpose, forty untrained, healthy and non-smoker male students (age=21.3±1.5 yrs, height=176±5.4 cm, and weight=74.2±14 kg) were divided into 4 groups  1) vitamin E + aerobic training; 2) aerobic training + placebo; 3) vitamin E; 4) placebo.
The research design involved vitamin E (400 mg per day) and aerobic training with 50-75% HRR (three days per week), and incremental cycle ergometer test until exhaustion. Before and after program, blood samples were collected at rest and post-exhaustive exercise. Also, blood lactate was measured by lactometer. ANOVA-one way, Tukay post hoc and Pearson correlation coefficient were used for analysis of data.
The results showed that vitamin E supplementation and aerobic training induced significant differences between groups in blood lactate and exhaustion and did notlead to significant difference in LDH enzyme. Also there was not significant correlation between groups in variables, except blood lactate and LDH enzyme in placebo group.
The results show that 8 weeks vitamin E supplementation and aerobic training cincided with beneficial changes in blood lactate and exhaustion, but can not change LDH enzyme profile of sedentary men. Thus, accumulation of blood lactate can not be the main reason of exhaustion in aerobic training and probably, other mechanisms play more important role in this case.

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References

. ابراهیم، خسرو. (1377). اثر تخریبی رادیکال‌های آزاد در هنگام فعّالیّت‌های شدید و خسته‌‌کننده و هم‌چنین نقش ویتامین‌ها و آنزیم‌های ضداکسیداتیو. المپیک، 1: 19-22.
2. گایینی، عبّاس‌علی و ظفری، اردشیر. (1384). مقایسة دو برنامة بازگشت به حالت اوّلیّه (فعّال و غیر فعّال) بر تغییرات لاکتات خون ناشی از یک فعّالیّت شدید درمانده ساز. المپیک، 32: 21-30.
3. رمضانی، علی‌رضا، نیک‌بخت، حجت‌الله و امیرتاش، محمّدعلی. (1382). تأثیر روش‌های بازیافت فعّال و غیر فعّال بر سطح لاکتات خون و ضربان قلب پس از فعّالیّت شدید غیر هوازی در شناگران نخبه. المپیک، 23: 5-14.
4. نامنی، فرح، کاشف، مجید و لاری، علی‌اصغر. (1383). تأثیر گرم‌کردن بر رابطه CK و LDH در دوره بازیافت زنان ورزشکار. المپیک، 28: 97-107.   
5. ویلمور، جک. اچ و کاستیل، دیوید. ال. فیزیولوژی ورزش و فعّالیّت بدنی. ترجمة ضیا معینی، فرهاد رحمانی‌نیا، حمید رجبی، حمید آقاعلی‌نژاد و فاطمه سلامی. (1377). تهران، انتشارات مبتکران، ص‌ص: 182و183. 
6. Banerjee, A.K., A, Mandal, D, Chanda, and S, Chakroborti. (2003). Oxidant, antioxidant and physical exercise. Molecular and Cellular Biochemistry. 253: 307-312.
7. Chevion, S., D.S, Morran, Y, Held, et al. (2003). Plasma antioxidant status and cell injury after sever physical exercise. Proc. Natl. Acad. Sci. 100(9): 5119-5123.
8. Clarkson, P.M., and H.S, Thompson. (2000). Antioxidants: what role do they play in physical activity and health? American Journal of Clinical Nutrition 72: 637-646.  
9. Helgheim, I., Q, Heltland, S, Nilsson, F, Ingjer, and S.B, Stromme. (1979). the effects of vitamin E on serum enzyme levels following heavy exercise. European Journal of Applied Physiology. 40: 283-289.    
10. Horita, T., N.C, Komi, C, Nicol, and H, Kyrolainen. (1999). Effect of exhausting stretch shorting cycle exercise on the time course of mechanical behavior in drop jump. European Journal of Applied Physiology and Occupation Physiology. 79: 160-167.   
11. Itoh, H., T, Ohkuwa, Y, Yamazaki, T, Shimoda, A, Wakayama, S, Tamura, T, Yamamoto, Y, Sato, and M, Miyamura. (2000). Vitamin E supplementation attenuates leakage of enzymes following 6 successive days of running training. International Journal of Sports Medicine. 21(5): 369-74.    
12. Lesgard, J.F., P, Durand, M, Larssarre, P, Stocker, G, Lesgards, a, Lanteaume, M, Prost, and M.P, Lehucher-Michel. (2002). Assessment of lifestyle effects on the overral antioxidant capacity of healthy subjects. Environment health prescriptive. 110: 479-486.
13. Radak, Z., K, Asano., M, Inoue., T, Kizaki., S, Oh-Ishi., K, Suzuki., N. Taniguchi., and H, Ohno. (1996). Superoxide desimutase derivative prevents oxidative damage in liver and kidney of rats induced by exhausting exercise. European Journal of Applied Physiology and Occupation Physiology. 72(3): 189-194.    
14. Rokitzki, L., E, Logemann, G, Huber, E, Keck, and J, Keul. (1994). Alpha-tocopherol Supplementation in racing cyclists during extreme endurance training. International Journal of Sport Nutritio. 4: 253-261.     
15. Sacheck, J. M, and J.B, Blumberg. (2001). Role of vitamin E and oxidative stress in exercise. Nutrition. 17: 809-814.    
16. Schantz, P.G. (1998). Plasticity of human skeletal muscle with special reference to effects of physical training on enzyme levels. Acta Physiologica Scandinavica. Supplementum. 558:1-62. 
17. Spriet, L.L., R.A, Howlett, and G.J.F, Heigenhauser. (2000). An enzymatic approach to lactate production in human skeletal muscle during exercise. Medicine and Science in Sports and Exercise. 32: 756-763. 
18. Tiidus, P.M., J, Pushkarenko, and M.E, Houston. (1996). Lack of antioxidant adaptation to short-term aerobic training in human muscle. American Journal of Physiology. 571(4Pt2): R832-836.
19. Tonkonogi, M., Walsh, B, Svensson, M, and Sahlin, K. (2000). Mitochondrial function and antioxidative defense in human muscle: effects of endurance training and oxidative stress. Journal of Physiology. 528(2): 379-388
 
Journal of Applied Exercise Physiology
Volume 2, Issue 4 - Serial Number 4
December 2006
Pages 59-69

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History

  • Receive Date: 22 October 2006
  • Revise Date: 05 November 2016
  • Accept Date: 14 January 2007

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