Presentation of Lacto-Resistance Training Method and Comparing Its Effect on Muscle Hypertrophy with Traditional Resistance Training In Professional Bodybuilders

Document Type : Research Paper

Authors

1 Department of Exercise physiology, Faculty of Sport Sciences, Shahid Bahonar University of Kerman, Kerman, Iran

2 Clinical Research Unit, Afzalipour Haspital, Kerman University of Medical Sciences, Kerman, Iran

Abstract

Background & Purpose: Manipulating of training variables with the aim to augment muscle metabolic stress could result in muscle hypertrophy.The purpose of the current study was to present a lacto-resistance training method and to compare its effect on rectus femoris muscle hypertrophy with traditional resistance training.
Methodology: Twenty- four male professional bodybuilders were randomly divided into three groups based on muscle cross-section area: lacto-resistance (n=8), traditional-resistance (n=8) and control (n=8). The comparison of metabolic stress between two types of exercise was done using monitoring blood lactate and urea following squat exercise. Muscle cross-section area and strength were measured by sonography and one repetition maximum (1RM), respectively, before and after 4 weeks of resistance training.  Analysis of covariance was used to evaluate inter – and intra groups differences.
Results: Compared to the baseline values, blood lactate levels significantly increased following squat exercise in both lacto-resistance (10.76±1.35) and traditional–resistance (7.70±1.16) groups, and the changes in blood lactate was significant between two groups (P˂0.05). After 4 weeks of resistance training, the changes in rectus femoris muscle cross-section had a significant difference only in lacto-resistance group in comparison to its own baseline values (P˂0.05) and the control group (P˂0.05). Compared to the baseline values, 1RM measured in squat exercise significantly increased in both experimental groups (P˂0.01), the difference between groups was significant, and lacto-resistance group had higher values than those from the traditional-resistance group (P˂0.05).
Conclusion: Compared to the traditional resistance training, Lacto-resistance training induces a higher metabolic stress and its effects on muscle hypertrophy and strength are more impressive.

Keywords


##Maughan, RJ, Watson, JS, Weir, J, 1983, Strength and cross-section area of human skeletalmuscle, J physiol, 338, 37-49.##Toigo, M, Boutellier, U, 2006, New fundamental resistance exercise determinants of molecular and cellular muscle adaptations, APPL physiol, 97, 643-63.##Damas, F, Phillips, S, Vechin, FC, Ugrinowitsch, C, 2015, A review of resistance training-induced changes in skeletal muscle protein synthesis and their contribution to hypertrophy, Sports Med, 45, 801-807.##Ricardo, C, Ismair, T, Daniel, V, Jansen, F, Sergio, T, Marco, T, 2013, Animal model for progressive resistance exercise: a detailed description of model and its implications for basic research in exercise, Motriz Rio Claro, 34, 305-308.##Schoenfeld, BJ, 2010, The mechanisms of muscle hypertrophy and their application to resistance training, J Strength Cond Res, 24, 2857-2872.##Michel, RN , Dunn, SE, Chin, ER, 2004, Calcineurin and skeletal muscle growth, Proc Nutr Soc, 63, 341–349.##Bodine, SC, Stitt, TN, Gonzalez, M, Kline, WO, Stover, GL, Bauerlein, R, Zlotchenko, E, Scrimgeour, A, Lawrence, JC, Glass, DJ, Yancopoulos, GD, 2001, Akt/mTOR pathway is a crucial regulator of skeletal muscle hypertrophy and can prevent muscle atrophy in vivo, Nat Cell Biol, 3, 1014–1019.##Kramer, HF, Goodyear, LJ, 2007, Exercise MAPK and NF-kappaB signaling in skeletal muscle, J Appl Physiol, 103,388–395.##Egan, B, Zierath, JR, 2013, Exercise metabolism and the molecular regulation of skeletal muscle adaptation, Cell Metab, 17,162-184.##Volianitis, S, Secher, NH, Quistorff, B, 2010, The intracellular to extracellular proton gradient following maximal whole body exercise and its implication for anaerobic energy production, Eur J Appl Physiol, 109, 1171-1177.##Lee, C, Sohn, A, Park, Y, Kang, K, Lee, M, Kang, M, Jang J, Yang, J, Hong, K, 2015, A lactate-induced response to hypoxia,Cell,161,595–609.##Henselmans, M, Schoenfeld, BJ,2014, The effect of inter-set rest intervals on resistance exercise-induced muscle hypertrophy, Sports Med, 44, 1635-1643.##Schott J, McCully K, Rutherford OM. 1995. The role of metabolites in strength training. II. Short versus long isometric contractions. Eur J Appl Physiol. 71: 337–341.##Gordon, SE, Kraemer, WJ, Vos, NH, Lynch, JM, Knuttgen, HG,1994, Effect of acid–base balance on the growth hormone response to acute high-intensity cycle exercise, J Appl Physiol, 76, 821–829.##Goto, K, Ishii, N, Kizuka, T,Takamatsu, K, 2005, The impact of metabolic stress on hormonal responses and muscular adaptations, Med Sci Sport Exerc, 37, 955–963.##Serrano, AL, Baeza, Raja, B, Perdiguero, E, 2008, Interleukin-6 is an essential regulator of satellite cell mediated skeletal muscle hypertrophy, Metab, 7, 33-44.##Yoshimi, O, Hayato, T, Takum, IY, Keisuke, H, mariko,- s, 2015, Mixedlactate and caffeine compound increases satellite cell activity and anabolic signals for muscle hypertrophy, J APPL physiol, 118: 742-9.##Kang, C, Zhang, Y, 2016, Exercise-induced hormesis and skeletal muscle health, Free Radic Biol Med,98, 113-122.##Gonzalez, AM, Hoffman, JR, 2015, Intramuscular anabolic signaling and endocrine response following high volume and high intensity resistance exercise protocols in trained men, Physiol Rep, 3, 12466.##Marchese, R, Hill, A, 2011, The essential guide to fitness: for the fitness instructor.Sydney nsw, 135, 978144.##Fink, J, Kikuchi, N, Yoshida, S, Terada, K, and Nakazato, K, Impact of high versus low fixed loads and non-linear training loads on muscle hypertrophy, strength and force development, Springerplus,2016, 5, 698-016.##Karp, J R, 2001, Muscle Fiber Types and Training, Track Coach, 155,4943-4946.##Nico, N, Lutz, B, Martin, W, Tilo, N, Michael, F, Henry, S, 2017, Acute effects of three resistance exercise programs on energy metabolism, International Journal of Sports Science,  7(2), 29-35.##Wilkinson, DJ, 2010, Am­monia metabolism the brain and fatigue revisiting the link, Prog, Neurobiol, 91, 200-219.##Felig, P, 1973, The glucose-alanine cycle, Metabolism,  22(2), 179-207.##Rogatzki, MJWright, GAMikat, RPBrice, AG, 2014, Blood ammonium and lactate accumulation response to different training protocols using the parallel squat exercise, J Strength Cond Res,  28(4),1113-8.##Peter, J, 1997, Ammonia and Lactate Response to Leg Press Work at 5 and 15 RM, Journal of Strength and Conditioning Research, 11, 40-44.##Lowenstein, JMGoodman, MN, 1978, the purine nucleotide cycle in skeletal muscle, Fed Proc, 37(9), 2308-12.##Malcolm, W, 2003, The urea cycle: Teaching intermediary metabolism in a physiological setting,IUBM Journal,31,289-297.##Marcelo, C, Jose, G, Nelo, E, Fabio, S, 2017, Role of metabolic stress for enhancing muscle adaptation, world journal of methodology, 7, 46-54.##Zhou , X, Naguro, I, Ichijo, H, Watanabe, K,2016, Mitogen-activated protein kinases as key players in osmotic stress signaling, Biochim Biophys Acta , 1860, 2037-2052.##Lang, F, 2007, Mechanisms and significance of cell volume regulation, J Am Coll Nutr, 26, 613-623.##Handayaningsih, AE, Iguchi, G, Fukuoka, H, Nishizawa, H, Takahashi, M, Yamamoto, M, Herningtyas, EH, Okimura, Y, Kaji H, Chihara, K, Seino, S, Takahashi, Y, 2011, Reactive oxygen species play an essential role in IGF-I signaling and IGF-I-induced myocyte hypertrophy in C2C12 myocytes, Endocrinology,152, 912-921.##Lena, W, aus, H, 2014, THE effects of lactate on skeletal muscle, Universität zu Köln, 4,55-65.##Takarada, Y, Nakamura, Y, Aruga, S, et al, 2000, Rapid increase in plasma growth hormone after low-intensity resistance exercise with vascular occlusion, J Appl Physiol,88(1),61–5.Nishimura, A, Sugita , M, Kato, K, Fukuda, A, Sudo, A, Uchida, A, 2010, Hypoxia increases muscle hypertrophy induced by resistance training, Int J Sports Physiol Perform, 5, 497-508.##Watanabe, Y, Madarame, H, Ogasawara, R, Nakazato, K, Ishii, N, 2014, Effect of very low-intensity resistance training with slow movement on muscle size and strength in healthy older adults, Clin Physiol Funct I, 34(6),463-470.##